pYES3/CT Yeast Expression Vector

Catalog number: V825320

Invitrogen™  Related applications: Protein Expression

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pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.
For Research Use Only. Not for use in diagnostic procedures.


Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: YES™
Product Size: 20 µg
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Vector Type: pYES

Contents & storage

Vectors are supplied supercoiled and lyophilized and include a lacZ expression control vector and an INVSc1 yeast stab. The pYES6/CT Starter Kit also includes 50 mg of Blasticidin. Store the vectors at -20°C. Store the INVSc1 stab and Blasticidin at +4°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols