Invitrogen™

pSecTag2 A, B, & C Mammalian Expression Vectors

Catalog number: V90020
Have Questions?
Invitrogen™

pSecTag2 A, B, & C Mammalian Expression Vectors

Catalog number: V90020
Have Questions?
Catalog Number
V90020
Unit Size
20 µg
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V9002020 µg
-
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pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features:

• Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2)
• Cytomegalovirus (CMV) promoter for high-level constitutive expression
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• C-terminal c-myc epitope for detection with an Anti-myc Antibody
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7)

The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.

The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Delivery Type
Transfection
Promoter
CMV
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Zeocin™
Protein Tag
His Tag (6x), c-Myc Epitope Tag, IgK Leader Sequence
Cloning Method
Restriction Enzyme/MCS
Vector
pSec
For Use With (Application)
Secreted Expression

Contents & Storage

20 μg of each vector and a PSA expression control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

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