Dysmorphic photoreceptors in a P23H mutant rhodopsin model of retinitis pigmentosa are metabolically active and capable of regenerating to reverse retinal degeneration.
AuthorsLee DC, Vazquez-Chona FR, Ferrell WD, Tam BM, Jones BW, Marc RE, Moritz OL,
JournalJ Neurosci
PubMed ID22323724
'This study evaluated the capacity of Xenopus laevis retina to regenerate photoreceptor cells after cyclic light-mediated acute rod photoreceptor degeneration in a transgenic P23H mutant rhodopsin model of retinits pigmentosa. After discontinuation of cyclic light exposure, we monitored histologic progression of retinal regeneration over a 3 week recovery period. To ... More
Phosphoglycerate mutase affects Stenotrophomonas maltophilia attachment to biotic and abiotic surfaces.
AuthorsRamos-Hegazy L, Chakravarty S, Anderson GG
Journal
PubMed ID31430538
Stenotrophomonas maltophilia biofilm formation is of increasing medical concern, particularly for lung infections. However, the molecular mechanisms facilitating the biofilm lifestyle in S. maltophilia are poorly understood. We generated and screened a transposon mutant library for mutations that lead to altered biofilm formation compared to wild type. One of these mutations, ... More
Axon sorting within the spinal cord marginal zone via Robo-mediated inhibition of N-cadherin controls spinocerebellar tract formation.
AuthorsSakai N, Insolera R, Sillitoe RV, Shi SH, Kaprielian Z,
JournalJ Neurosci
PubMed ID23115176
The axons of spinal projection neurons transmit sensory information to the brain by ascending within highly organized longitudinal tracts. However, the molecular mechanisms that control the sorting of these axons within the spinal cord and their directed growth to poorly defined targets are not understood. Here, we show that an ... More
The transmembrane domain of the severe acute respiratory syndrome coronavirus ORF7b protein is necessary and sufficient for its retention in the Golgi complex.
AuthorsSchaecher SR, Diamond MS, Pekosz A,
JournalJ Virol
PubMed ID18632859
The severe acute respiratory syndrome coronavirus (SARS-CoV) ORF7b (also called 7b) protein is an integral membrane protein that is translated from a bicistronic open reading frame encoded within subgenomic RNA 7. When expressed independently or during virus infection, ORF7b accumulates in the Golgi compartment, colocalizing with both cis- and trans-Golgi ... More
A novel celecoxib analog UTX-121 inhibits HT1080 cell invasion by modulating membrane-type 1 matrix metalloproteinase.
AuthorsYamahana H, Takino T, Endo Y, Yamada H, Suzuki T, Uto Y
JournalBiochem Biophys Res Commun
PubMed ID31629465
'We designed and synthesized a celecoxib derivative UTX-121 to enhance its anti-tumor activity. Similar to celecoxib, this compound could also inhibit matrix metalloproteinase (MMP)-9 activity. In addition, UTX-121 suppressed membrane-type 1 MMP (MT1-MMP)-mediated pro-MMP-2 activation by disturbing the cell surface expression of MT1-MMP. UTX-121 also impeded the glycosylation of cell ... More
AuthorsHe J, Li T, Próchnicki T, Horvath G, Latz E, Takeoka S
JournalBiochem Biophys Rep
PubMed ID31011633
'Lysine (K) type cationic lipid with a propyl spacer and ditetradecyl hydrophobic moieties composing liposomes, K3C14, previously studied for gene delivery, were reported to activate the NLRP3 inflammasomes in human macrophages via the conventional phagolysosomal pathway. In this study, K3C16, a propyl spacer bearing lysine type lipids with dihexadecyl moieties ... More
TRPV1 activity and substance P release are required for corneal cold nociception.
Authors
JournalNat Commun
PubMed ID31831729
Neuromodulation of the cerebellum rescues movement in a mouse model of ataxia.
Authors
JournalNat Commun
PubMed ID33637754
Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity.
Authors
JournalNat Cell Biol
PubMed ID21909099
NOTUM from Apc-mutant cells biases clonal competition to initiate cancer.
Authors
JournalNature
PubMed ID34079124
IRF1-mediated downregulation of PGC1α contributes to cardiorenal syndrome type 4.
Authors
JournalNat Commun
PubMed ID32938919
The origin of heterogeneous nanoparticle uptake by cells.
Authors
JournalNat Commun
PubMed ID31138801
Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection.
Authors
JournalCell Host Microbe
PubMed ID31492657
Integrating deep learning and unbiased automated high-content screening to identify complex disease signatures in human fibroblasts.
Authors
JournalNat Commun
PubMed ID35338121
High spatial and temporal resolution Ca2+ imaging of myocardial strips from human, pig and rat.
Authors
JournalNat Protoc
PubMed ID34400840
Control of osteocyte dendrite formation by Sp7 and its target gene osteocrin.
Authors
JournalNat Commun
PubMed ID34725346
A simple and robust fluorescent labeling method to quantify trophoblast fusion.
AuthorsZhang Y, Yang H
JournalPlacenta
PubMed ID30827351
Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared ... More
Dynamic Transcriptional Responses to Injury of Regenerative and Non-regenerative Cardiomyocytes Revealed by Single-Nucleus RNA Sequencing.
AuthorsCui M, Wang Z, Chen K, Shah AM, Tan W, Duan L, Sanchez-Ortiz E, Li H, Xu L, Liu N, Bassel-Duby R, Olson EN
JournalDev Cell
PubMed ID32220304
The adult mammalian heart is incapable of regeneration following injury. In contrast, the neonatal mouse heart can efficiently regenerate during the first week of life. The molecular mechanisms that mediate the regenerative response and its blockade in later life are not understood. Here, by single-nucleus RNA sequencing, we map the ... More
Glycine Enhances Satellite Cell Proliferation, Cell Transplantation, and Oligonucleotide Efficacy in Dystrophic Muscle.
AuthorsLin C, Han G, Ning H, Song J, Ran N, Yi X, Seow Y, Yin H
JournalMol Ther
PubMed ID32209436
The need to distribute therapy evenly systemically throughout the large muscle volume within the body makes Duchenne muscular dystrophy (DMD) therapy a challenge. Cell and exon-skipping therapies are promising but have limited effects, and thus enhancing their therapeutic potency is of paramount importance to increase the accessibility of these therapies ... More
Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps.
AuthorsTanaka M, Kinoshita-Daitoku R, Kiga K, Sanada T, Zhu B, Okano T, Aikawa C, Iida T, Ogura Y, Hayashi T, Okubo K, Kurosawa M, Hirahashi J, Suzuki T, Nakagawa I, Nangaku M, Mimuro H
JournalSci Rep
PubMed ID32094510
Group A Streptococcus (GAS) secretes deoxyribonucleases and evades neutrophil extracellular killing by degrading neutrophil extracellular traps (NETs). However, limited information is currently available on the interaction between GAS and NETs in the pathogenicity of GAS pharyngitis. In this study, we modified a mouse model of GAS pharyngitis and revealed an ... More
Ovine Herpesvirus 2 Encodes a Previously Unrecognized Protein, pOv8.25, That Targets Mitochondria and Triggers Apoptotic Cell Death.
AuthorsShrestha N, Tobler K, Uster S, Sigrist-Nagy R, Hierweger MM, Ackermann M
JournalJ Virol
PubMed ID32024777
Malignant catarrhal fever (MCF) is a rare but frequently lethal disease of certain cloven-hoofed animals. At least 10 different viruses, all members of the
The HopQ-CEACAM Interaction Controls CagA Translocation, Phosphorylation, and Phagocytosis of
AuthorsBehrens IK, Busch B, Ishikawa-Ankerhold H, Palamides P, Shively JE, Stanners C, Chan C, Leung N, Gray-Owen S, Haas R
JournalmBio
PubMed ID32019805
The
Skeletal muscle lipid droplets are resynthesized before being coated with perilipin proteins following prolonged exercise in elite male triathletes.
AuthorsJevons EFP, Gejl KD, Strauss JA, Ørtenblad N, Shepherd SO
JournalAm J Physiol Endocrinol Metab
PubMed ID31935113
Intramuscular triglycerides (IMTG) are a key substrate during prolonged exercise, but little is known about the rate of IMTG resynthesis in the postexercise period. We investigated the hypothesis that the distribution of the lipid droplet (LD)-associated perilipin (PLIN) proteins is linked to IMTG storage following exercise. Fourteen elite male triathletes ... More
Multinucleated polyploid cardiomyocytes undergo an enhanced adaptability to hypoxia via mitophagy.
AuthorsJiang YH, Wang HL, Peng J, Zhu Y, Zhang HG, Tang FQ, Jian Z, Xiao YB
JournalJ Mol Cell Cardiol
PubMed ID31783035
There is a large subpopulation of multinucleated polyploid cardiomyocytes (M*Pc CMs) in the adult mammalian heart. However, the pathophysiological significance of increased M*Pc CMs in heart disease is poorly understood. We sought to determine the pathophysiological significance of increased M*Pc CMs during hypoxia adaptation. ... More