Invitrogen™

Wheat Germ Agglutinin (WGA)

Catalog number: W32464
Have Questions?
Invitrogen™

Wheat Germ Agglutinin (WGA)

Catalog number: W32464
Have Questions?

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates. These lectins can bind to carbohydrates and are available conjugated to Alexa Fluor™, Alexa Fluor™ Plus, and other fluorescent dyes. Fluorescent wheat germ agglutinin conjugates are valuable tools in molecular and cell biology research, enabling researchers to label the plasma membrane in fluorescence imaging and cell painting assays, and study and analyze glycosylation patterns and glycan-mediated processes in cells and tissues.

 
Catalog Number
W32464
Unit Size
5 mg
Conjugate
Alexa Fluor 555
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 555 Conjugate
ConjugateAlexa Fluor 555
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 350
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 350 Conjugate
ConjugateAlexa Fluor 350
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
1 kit
Conjugate
Alexa Fluor 350
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity1 mg
SourceWheat germ
ConjugateAlexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
SpeciesWheat
RecombinantRecombinant
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 kit
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 488
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 488 Conjugate
ConjugateAlexa Fluor 488
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 594
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 594 Conjugate
ConjugateAlexa Fluor 594
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 633
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 633 Conjugate
ConjugateAlexa Fluor 633
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 647
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 647 Conjugate
ConjugateAlexa Fluor 647
SpeciesWheat
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
5 mg
Conjugate
Alexa Fluor 680
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 680 Conjugate
ConjugateAlexa Fluor 680
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
Catalog Number
Unit Size
1 mg
Conjugate
Alexa Fluor Plus 405
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Common NameWheat Germ Agglutinin, Alexa Fluor™ Plus 405 Conjugate
ConjugateAlexa Fluor Plus 405
SpeciesWheat
RecombinantNative
Protein TagNone
Excitation/Emission405/450 nm
Flow Cytometer Laser LinesUV/Violet, 405 nm
Sub Cellular LocalizationPlasma Membrane
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormSolid
Protein SubtypeAgglutinin
ColorViolet
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 mg
Catalog Number
Unit Size
1 mg
Conjugate
Alexa Fluor Plus 568
Price (USD)
Availability
-
Full specifications
Quantity
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Common NameWheat Germ Agglutinin, Alexa Fluor™ Plus 568 Conjugate
ConjugateAlexa Fluor Plus 568
SpeciesWheat
RecombinantNative
Protein TagNone
Excitation/Emission562/583 nm
Flow Cytometer Laser LinesTexas Red, 568 nm
Sub Cellular LocalizationPlasma Membrane
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormSolid
Protein SubtypeAgglutinin
ColorRed
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 mg
Showing 10 of 16Show more
Catalog NumberSpecificationsUnit SizeConjugatePrice (USD)AvailabilityQuantity
W32464Full specifications
5 mgAlexa Fluor 555
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 555 Conjugate
ConjugateAlexa Fluor 555
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W11263Full specifications
5 mgAlexa Fluor 350
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 350 Conjugate
ConjugateAlexa Fluor 350
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W7024Full specifications
1 kitAlexa Fluor 350
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity1 mg
SourceWheat germ
ConjugateAlexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
SpeciesWheat
RecombinantRecombinant
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 kit
W11261Full specifications
5 mgAlexa Fluor 488
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 488 Conjugate
ConjugateAlexa Fluor 488
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W11262Full specifications
5 mgAlexa Fluor 594
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 594 Conjugate
ConjugateAlexa Fluor 594
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W21404Full specifications
5 mgAlexa Fluor 633
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 633 Conjugate
ConjugateAlexa Fluor 633
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W32466Full specifications
5 mgAlexa Fluor 647
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 647 Conjugate
ConjugateAlexa Fluor 647
SpeciesWheat
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W32465Full specifications
5 mgAlexa Fluor 680
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Quantity5 mg
SourceWheat germ
Common NameWheat Germ Agglutinin, Alexa Fluor™ 680 Conjugate
ConjugateAlexa Fluor 680
SpeciesWheat
RecombinantNative
Protein TagNone
Shipping ConditionRoom Temperature
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormLyophilized
Protein SubtypeAgglutinin
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size5 mg
W56132Full specifications
1 mgAlexa Fluor Plus 405
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Common NameWheat Germ Agglutinin, Alexa Fluor™ Plus 405 Conjugate
ConjugateAlexa Fluor Plus 405
SpeciesWheat
RecombinantNative
Protein TagNone
Excitation/Emission405/450 nm
Flow Cytometer Laser LinesUV/Violet, 405 nm
Sub Cellular LocalizationPlasma Membrane
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormSolid
Protein SubtypeAgglutinin
ColorViolet
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 mg
W56133Full specifications
1 mgAlexa Fluor Plus 568
-
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Common NameWheat Germ Agglutinin, Alexa Fluor™ Plus 568 Conjugate
ConjugateAlexa Fluor Plus 568
SpeciesWheat
RecombinantNative
Protein TagNone
Excitation/Emission562/583 nm
Flow Cytometer Laser LinesTexas Red, 568 nm
Sub Cellular LocalizationPlasma Membrane
Expression SystemWheat germ
Protein FamilyLectins
Protein FormHeterodimer
FormSolid
Protein SubtypeAgglutinin
ColorRed
Product LineAlexa Fluor™
Purity or Quality GradeSee Certificate of Analysis
ProteinFluorescent lectins
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Unit Size1 mg
Showing 10 of 16Show more

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) with Alexa Fluor, Alexa Fluor Plus, and other dyes. WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

These fluorescent lectin conjugates can also be used to label fixed cells; however, to avoid labeling intracellular components, formaldehyde-fixed cells should not be permeabilized before labeling. Fluorescent WGA conjugates are used as plasma membrane stains along with other cellular markers in cell painting assays to provide a phenotypic readout of cell health or cytotoxicity. The Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate is included in the Image-iT Cell Painting Kit (Cat. Nos. I65000 and I65500).

WGA conjugates are also used as retrograde tracers for neuronal tracing experiments and have been shown to cross synapses. These fluorescent lectins are applicable in microbiology studies to label yeast bud scars, the cell membrane of gram-positive but not gram-negative bacteria, and chitin in fungal cell walls. In solution, WGA exists as a heterodimer with a molecular weight of approximately 38,000 Daltons and is normally cationic under physiological conditions. Our WGA conjugates have been used in variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), and a wide range of chemical, biochemical and immunological assays.

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates with options covering the entire wavelength range. The Wheat Germ Agglutinin Sampler Kit (Cat. No. W7024) includes introductory samples of four fluorescent WGAs: Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine, and Texas Red-X conjugates. The red-fluorescent Alexa Fluor 594 wheat germ agglutinin (WGA) conjugate is also included in the Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit (Cat. No. I34406). The Texas Red-X WGA conjugate can be purchased in the ViaGram Red+ Bacterial Gram Stain and Viability Kit (Cat. No. V7023) to differentiate gram-positive and gram-negative bacteria.

Wheat germ agglutinin (WGA) conjugates fluorescence excitation/emission
W11261 WGA, Alexa Fluor 488 conjugate: 495 nm/519 nm
W11263 WGA, Alexa Fluor 350 conjugate: 346 nm/442 nm
W32464 WGA, Alexa Fluor 555 conjugate: 555 nm/580 nm
W11262 WGA, Alexa Fluor 594 conjugate: 590 nm/617 nm
W21404 WGA, Alexa Fluor 633 conjugate: 632 nm/647 nm
W32466 WGA, Alexa Fluor 647 conjugate: 650 nm/665 nm
W32465 WGA, Alexa Fluor 680 conjugate: 679 nm/702 nm
W56132 WGA, Alexa Fluor Plus 405 conjugate: 408 nm/450 nm
W56133 WGA, Alexa Fluor Plus 568 conjugate: 562 nm/583 nm
W56134 WGA, Alexa Fluor Plus 770 conjugate: 770 nm/797 nm
W834 WGA, fluorescein conjugate: 494 nm/518 nm
W6748 WGA, Oregon Green 488 conjugate: 496 nm/524 nm
W849 WGA, tetramethylrhodamine conjugate: 555 nm/580 nm
W21405 WGA, Texas Red-X conjugate: 595 nm/615 nm

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

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