Invitrogen™

pHrodo™ Deep Red Antibody Labeling Kit

Catalog number: P35355
Invitrogen™

pHrodo™ Deep Red Antibody Labeling Kit

Catalog number: P35355
Catalog Number
P35355
Unit Size
3 x 100 µg reactions
Price (USD)
Price: 646.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
3 x 1 mg reactions
Price (USD)
Price: 664.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
P353553 x 100 µg reactions
Price: 646.00
Your Price:
-
P353563 x 1 mg reactions
Price: 664.00
Your Price:
-
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The pHrodo Deep Red Antibody Labeling kit provides the materials needed to easily label and purify antibodies for the study of antibody internalization. Amine-reactive pHrodo Deep Red dye covalently binds to antibodies to produce bright, soluble conjugates that only fluoresce in acidic environments. The pHrodo Deep Red labeling kit makes it easier than ever to study antibody internalization with higher certainty of results and less optimization since pHrodo Deep Red dye only fluoresces in late endosomes and lysosomes.

pHrodo Deep Red Antibody Labeling Kit features include:
Superior signal window—minimal background at neutral pH enables maximal signal-to-noise ratio for certainty of results
Multiplex—Cy5 channel allows for multicolor experiments
Low pKa—eliminates non-specific signal from non-internalized cargo and only fluoresces in late endosomes and lysosomes
Convenient—labeling typically takes 2 hours with less than 30 min hands-on time

pHrodo Deep Red is a low-background pH sensor dye that shows no signal in neutral conditions and only fluoresces in acidic environments. This unique property helps enable rapid assay development and helps increase certainty of results when investigating antibody internalization and endocytic/phagocytic pathways. pHrodo Deep Red dye helps enable better discrimination of internalized cargo from outside the cell because it has an approximate pKa of 5 and does not fluoresce until it enters the late endosome and lysosome. pHrodo Deep Red dye can be detected using a Cy5 fluorescent filter set and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, and high content analysis (HCA).

Learn more about pHrodo dyes and antibody internalization.

Learn more about antibody labeling ›

Specifications

Product Type
Antibody Labeling Kit
Quantity
3 x 100 μg Reactions
Detection Method
Fluorescent
Excitation/Emission
640/655
Labeling Scale
100 μg
Chemical Reactivity
Amine
Reactive Moiety
TFP Ester
Label or Dye
pHrodo™ Deep Red
Color
Deep Red
Labeling Target
Antibodies