Sandwich ELISA analysis of human Interferon Gamma (IFNG) was performed using a Human Interferon Gamma Colorimetric ELISA kit (Product # EHINFG) by loading 50 ul per well of Interferon Gamma Recombinant Protein (Product # SIFNG) in quadruplicate at 1000, 400, 160, 64, 25.6, and 0 pg/ml across a 3 ug/ml mouse anti-human IFNG (Product # M700A) pre-coated plate and incubating for 2 hours at room temperature. The plate was washed, then incubated with 50 ul per well of a mouse anti-human IFNG biotinylated antibody (Product # M701B) in quadruplic... View More

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Product Details

SIFNG

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.5 ng/mL

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

The human IFNgamma Standard is calibrated against NIAID IFNgamma Reference Standard lot #Gg23-901-530. One Thermo pg = 56 NIAID pg = 0.04 NIAID units.

Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at -20°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.

Sufficient For: ELISA standard curve with replicates or as a control in Western blots.

2500 pg/ml when reconstituted at the recommend volume.

Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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IFN gamma Recombinant Protein

IFN gamma Protein (SIFNG) in ELISA Std