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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr419) Polyclonal Antibody

          121 References
          View all (78) SRC antibodies

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          Cite Phospho-SRC (Tyr419) Polyclonal Antibody

          • Antibody Testing Data (2)
          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-SRC (Tyr419) Antibody (44-660G) in IHC (P)

          Immunohistochemistry analysis of SRC showing staining in the cytoplasm and membrane of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room te... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-SRC (Tyr419) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-SRC (Tyr419) Antibody in Western Blot (WB)
          Phospho-SRC (Tyr419) Polyclonal Antibody

          Product Details

          44-660G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 61 publications 61 publications

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:100
          -

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 16 publications 16 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 10 publications 10 publications

          Affinity Purification (AP)

          -
          View 7 publications 7 publications

          Miscellaneous PubMed (Misc)

          -
          View 9 publications 9 publications
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse

          Published species

          Bovine, C. elegans, Dog, Fruit fly, Human, Mouse, Pig, Rat, Tag, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 419. The sequence is conserved in mouse, rat, chicken and frog.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533714

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: c-src protein, C terminus; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; ORF; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-scr; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; RP5-823N20.1; src downstream region; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; Unknown; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma viral oncogene

          View more View less

          Gene Aliases: ASV; AW259666; c-SRC; p60-Src; PP60C-SCR; pp60c-src; SDR; SRC; SRC1; THC6

          View more View less

          UniProt ID: (Chicken) P00523, (Human) P12931, (Mouse) P05480

          View more View less

          Entrez Gene ID: (Chicken) 396442, (Human) 6714, (Mouse) 20779

          View more View less

          Function(s)
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding protein phosphatase binding heme binding growth factor receptor binding connexin binding protein kinase activity SH3/SH2 adaptor activity protein kinase C binding insulin receptor binding integrin binding protein C-terminus binding kinase activity enzyme binding kinase binding estrogen receptor binding SH2 domain binding ion channel binding ephrin receptor binding phosphoprotein binding hormone receptor binding scaffold protein binding cadherin binding involved in cell-cell adhesion nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding protein complex binding cell adhesion molecule binding transmembrane signal receptor scaffold/adaptor protein
          Process(es)
          cell cycle cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway cell differentiation osteoclast development peptidyl-tyrosine autophosphorylation regulation of cell proliferation innate immune response bone resorption progesterone receptor signaling pathway regulation of cell cycle negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway stimulatory C-type lectin receptor signaling pathway signal transduction signal complex assembly transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway central nervous system development cell proliferation response to mechanical stimulus response to virus response to acidic pH regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion platelet activation intracellular estrogen receptor signaling pathway forebrain development T cell costimulation protein destabilization response to nutrient levels positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of telomere maintenance via telomerase negative regulation of protein homooligomerization cellular response to insulin stimulus regulation of intracellular estrogen receptor signaling pathway positive regulation of integrin activation adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction cellular response to platelet-derived growth factor stimulus Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway response to drug response to hydrogen peroxide positive regulation of apoptotic process negative regulation of apoptotic process regulation of vascular permeability stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis regulation of bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway ephrin receptor signaling pathway oogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive regulation of small GTPase mediated signal transduction response to mineralocorticoid response to electrical stimulus negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization negative regulation of telomerase activity uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to lipopolysaccharide cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fatty acid cellular response to hypoxia cellular response to fluid shear stress regulation of podosome assembly positive regulation of podosome assembly positive regulation of protein serine/threonine kinase activity angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis regulation of caveolin-mediated endocytosis immune system process protein phosphorylation positive regulation of gene expression phosphorylation cell migration positive regulation of cell adhesion positive regulation of protein transport cellular response to transforming growth factor beta stimulus positive regulation of intracellular signal transduction
          It has to be done as per old AB suggested Products section.
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