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Invitrogen
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Description: The HIS36 monoclonal antibody reacts with a molecule expressed on the surface of rat macrophages. The antigen recognized by HIS36 is an ED2-like antigen expressed by thioglycolate-elicited peritoneal cells and tissue macrophages. This antibody is used as a marker for rat tissue macrophages.
Applications Reported: The HIS36 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The HIS36 antibody has been tested by flow cytometric analysis of rat spleen and peritoneal cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ED2-like antigen; Macrophages; Mature Macrophages
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