Invitrogen

SRSF1 Monoclonal Antibody (103)

Name: SRSF1 Monoclonal Antibody (103)
Brand: Invitrogen
SKU: 32-4600

Advanced Verification

27 Published Figures

38 References

View all (19) SRSF1 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Datasheet

Certificates

Protocols

Questions & Answers

Additional Information:

SRSF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 30

SRSF1 Antibody (32-4600) in ICC/IF

Immunofluorescence analysis of SF2/ASF was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SF2 / ASF (103) Mouse Monoclonal Antibody (Product # 32-4600) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # ... View More

SRSF1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

SRSF1 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

SRSF1 Antibody in Immunohistochemistry (IHC)

SRSF1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

SRSF1 Antibody — Figure 2 Inhibition of transcription enhances the interaction between RRP1B and SRSF1 A. Co-immunoprecipitation of RRP1B and SRSF1 with DRB or Act D treatment. 293T cells treated with DMSO, 25 mug/mL DRB, or 2.5 mug/mL and 5 mug/mL Act D as indicated were used for immunoprecipitation with anti-RRP1B. Normal rabbit IgG was used as a negative control. B. Co-immunofluorescence of RRP1B and SRSF1 after DMSO or 25 mug/mL DRB treatment. 293T cells were treated with 2 h DMSO or DRB and collected for immunofluorescence. Co-localization was confirmed using confocal fluorescence microscopy. Scale bar measures 10 muM. {TM}

Product Details

32-4600

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 14 publications 14 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

103

Immunogen

Recombinant SF2/ASF protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533080

Target Information

UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alternative; Alternative-splicing factor 1; ASF-1; ASF/SF2; OK/SW-cl.3; pre-mRNA-splicing factor SF2, P33 subunit; Pre-mRNA-splicing factor SRp30a; Serine/arginine-rich splicing factor 1; splicing factor 2; Splicing factor, arginine/serine-rich 1; splicing factor, arginine/serine-rich 1 (ASF/SF2); splicing factor, arginine/serine-rich 1B; splicing factor, arginine/serine-rich, 30-KD, A; SR splicing factor 1; unnamed protein product

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Gene Aliases: 1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; ASF; AW491331; NEDFBA; OK/SW-cl.3; SF2; SF2P33; SFRS1; SRp30a; SRSF1

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UniProt ID: (Human) Q07955, (Mouse) Q6PDM2

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Entrez Gene ID: (Human) 6426, (Rat) 689890, (Mouse) 110809

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Function(s)

nucleic acid binding RNA binding mRNA binding protein binding protein kinase B binding DNA topoisomerase binding RS domain binding nucleotide binding poly(A) RNA binding RNA splicing factor

Process(es)

alternative mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome mRNA 5'-splice site recognition mRNA splicing, via spliceosome in utero embryonic development mRNA splice site selection mRNA processing RNA splicing signal transduction involved in regulation of gene expression positive regulation of RNA splicing protein localization to nucleus regulation of RNA splicing mRNA stabilization oligodendrocyte differentiation mRNA transport cardiac muscle contraction cellular response to interleukin-17 interleukin-17-mediated signaling pathway liver regeneration protein localization to P-body regulation of transcription, DNA-templated regulation of translation regulation of mRNA stability regulation of mRNA splicing, via spliceosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SRSF1 Monoclonal Antibody (103)

SRSF1 Antibody (32-4600) in ICC/IF

Product Details

32-4600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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