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Invitrogen
CRHR1 Recombinant Rabbit Monoclonal Antibody (4H5L2)
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FIGURE: 1 / 4
CRHR1 Antibody (702611) in ICC/IF
For immunocytochemistry analysis, HEK 293 cells stably transfected with CRHR1 were grown on poly-L-lysine-coated coverslips overnight. Cells were then treated with 1 µM Corticotropin releasing hormone for 30 min, washed with PBS and fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-CRHR1 Recombinant Rabbit Monoclonal Antibody (Product # 702611, 1 µg/mL... View More
Product Details
702611
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4H5L2
Immunogen
Peptide corresponding to human CRHR1 [aa425-aa444]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Pig, Rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing. CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: corticotrophin releasing hormone receptor 1; corticotropin releasing hormone 1; Corticotropin-releasing factor receptor 1; corticotropin-releasing factor receptor variant 1j; corticotropin-releasing factor type 1 receptor; Corticotropin-releasing hormone receptor 1; Crf receptor 1; Crf type 1; CRF-R; CRF-R-1; CRF-R1; CRF1; Crf1 receptor; CRF1I; CRFR-1; CRH-R 1; CRH-R-1; CRH-R1; CRH-R1d; type 1 CRH receptor; CRH-R1h; CRHR1e; CRHR1f; CRHR1g; G protein-coupled receptor; seven transmembrane helix receptor
Gene Aliases: CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1; CRHR1L
UniProt ID: (Human) P34998, (Rat) P35353
Entrez Gene ID: (Human) 1394, (Rat) 58959
transmembrane signaling receptor activity
G-protein coupled receptor activity
protein binding
G-protein coupled peptide receptor activity
corticotrophin-releasing factor receptor activity
corticotropin-releasing hormone receptor activity
corticotropin-releasing hormone binding
G-protein alpha-subunit binding
protein complex binding
peptide binding
transmembrane signal receptor
immune response
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
female pregnancy
parturition
hormone-mediated signaling pathway
adrenal gland development
cellular response to hormone stimulus
exploration behavior
fear response
behavioral response to ethanol
regulation of transport
corticotropin secretion
general adaptation syndrome, behavioral process
cellular response to corticotropin-releasing hormone stimulus
negative regulation of voltage-gated calcium channel activity
regulation of corticosterone secretion
response to hypoxia
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
neuropeptide signaling pathway
memory
feeding behavior
visual learning
regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
hypothalamus development
positive regulation of cAMP biosynthetic process
epithelial cell differentiation
negative regulation of epinephrine secretion
locomotory exploration behavior
response to immobilization stress
positive regulation of mast cell degranulation
behavioral response to cocaine
regulation of synaptic plasticity
behavioral response to pain
response to electrical stimulus
long-term synaptic potentiation
negative regulation of neuron death
negative regulation of feeding behavior
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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