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Invitrogen
BHLHE41 Recombinant Rabbit Monoclonal Antibody (1H18L6)
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FIGURE: 1 / 4
BHLHE41 Antibody (702917) in WB
Knockdown of BHLH3 was achieved by transfecting MDA-MB-231 cells with BHLH3 specific siRNA (Silencer® select Product # s35755). Western blot analysis (Fig a) was performed using modified whole cell extracts (1% SDS) from BHLH3 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-BHLH3 Recombinant Rabbit Monoclonal Antibody (Product # 702917, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Prod... View More
Product Details
702917
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H18L6
Immunogen
Protein corresponding to human BHLH3 [aa246-aa482]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The BHLHE41 gene encodes a basic helix-loop-helix (bHLH) transcription factor located at the 12p12.1 susceptibility locus. It functions primarily as a nuclear transcriptional repressor, homodimerizing or heterodimerizing with various partners to regulate gene expression. BHLHE41 expression is highly tissue-specific and responsive to environmental cues. Genetic studies have highlighted its role in increasing xenograft tumor growth and its involvement in short sleep phenotypes. A notable mutation in BHLHE41 leads to a phenotype requiring only six hours of sleep, demonstrating its influence on sleep patterns. Moreover, BHLHE41 has been associated with osteoclast differentiation processes, indicating its involvement in cellular development. Its interaction with TFEB target genes suggests a protective role against certain types of cell death, emphasizing its complex role in cellular physiology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-Dec; basic helix-loop-helix domain containing, class B, 3; bHLHb3; bHLHe41; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1; hDEC2; SHARP-1; unnamed protein product
Gene Aliases: BHLHB3; BHLHE41; DEC2; FNSS1; hDEC2; SHARP1
UniProt ID: (Human) Q9C0J9
Entrez Gene ID: (Human) 79365
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
MRF binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
circadian rhythm
anterior/posterior pattern specification
negative regulation of myotube differentiation
negative regulation of transcription by competitive promoter binding
circadian regulation of gene expression
negative regulation of transcription, DNA-templated
rhythmic process
regulation of neurogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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