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Invitrogen

ERK2 Monoclonal Antibody (6F8)

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View all (82) ERK2 antibodies

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Product Details

MA1-099

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:200
-

Flow Cytometry (Flow)

1-10 µg/mL
-

Immunoprecipitation (IP)

2 µg/500 µL
-

ChIP assay (ChIP)

1-3 µL
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6F8

Immunogen

Protein expressed in 293T cells transfected with human MAPK1 expression vector

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536743

Product Specific Information

MA1-099 has been successfully used in Western blot, ChIP, flow cytometry, immunofluorescence, immunohistochemistry and immunoprecipitation applications, and reacts with human, dog, rat, mouse and monkey samples.

MA1-099 has successfully been used in western blot to detect p42 ERK in HeLa, NRK and MDCK cell lysates.

As demonstrated by a Western blot using recombinant ERK1 and ERK2 proteins, MA1-099 can also detect ERK1, but detects ERK2 at much lower concentrations. As demonstrated by a Western blot using a panel of lysates from various species, MA1-099 only detects endogenous ERK2.

Target Information

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ERK-2; Erk2; cancer susceptibility protein; ERT1; Extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2; unnamed protein product

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Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-2; ERK2; ERT1; Mapk; MAPK1; MAPK2; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2

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UniProt ID: (Human) P28482, (Mouse) P63085, (Rat) P63086

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Entrez Gene ID: (Human) 5594, (Dog) 477575, (Mouse) 26413, (Rat) 116590

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Function(s)
nucleotide binding phosphotyrosine binding DNA binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity transferase activity phosphatase binding identical protein binding protein serine kinase activity transcription factor binding transferase activity, transferring phosphorus-containing groups protein kinase binding mitogen-activated protein kinase kinase kinase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade regulation of transcription from RNA polymerase II promoter protein phosphorylation apoptotic process chemotaxis cellular response to DNA damage stimulus signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway chemical synaptic transmission heart development learning or memory insulin receptor signaling pathway organ morphogenesis positive regulation of macrophage chemotaxis positive regulation of peptidyl-threonine phosphorylation neural crest cell development Schwann cell development peptidyl-threonine phosphorylation cytosine metabolic process regulation of ossification regulation of cellular pH thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance response to lipopolysaccharide regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation cellular response to amino acid starvation response to nicotine intracellular signal transduction ERBB signaling pathway ERBB2-ERBB3 signaling pathway outer ear morphogenesis myelination response to exogenous dsRNA positive regulation of cholesterol biosynthetic process negative regulation of cell differentiation insulin-like growth factor receptor signaling pathway thymus development T cell receptor signaling pathway B cell receptor signaling pathway stress-activated MAPK cascade regulation of cytoskeleton organization Bergmann glial cell differentiation long-term synaptic potentiation face development lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development interleukin-34-mediated signaling pathway chemokine-mediated signaling pathway ERK1 and ERK2 cascade response to epidermal growth factor cellular response to tumor necrosis factor caveolin-mediated endocytosis regulation of Golgi inheritance positive regulation of macrophage proliferation positive regulation of neuroinflammatory response regulation of early endosome to late endosome transport MAPK import into nucleus transcription, DNA-templated cell cycle response to toxic substance phosphorylation peptidyl-serine phosphorylation sensory perception of pain response to estrogen positive regulation of translation regulation of sequence-specific DNA binding transcription factor activity cellular response to organic substance cellular response to granulocyte macrophage colony-stimulating factor stimulus positive regulation of cell proliferation positive regulation of cell migration positive regulation of transcription, DNA-templated
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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