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          • Primary Antibodies ›
          • beta Galactosidase Antibodies

          Invitrogen

          beta Galactosidase Monoclonal Antibody (DC1 4C7)

          1 Published Figure
          3 References
          View all (49) beta Galactosidase antibodies

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          Cite beta Galactosidase Monoclonal Antibody (DC1 4C7)

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (1)
          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
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          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          beta Galactosidase Antibody (MA1-152) in ICC/IF

          Immunofluorescent analysis of E. coli beta-Galactosidase (green) in pCMV-LacZ transfected HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a beta-Galactosidase monoclonal antibody (Product # MA1-152) at a dilution of 1:50 for... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          beta Galactosidase Antibody in Immunocytochemistry (ICC/IF)
          beta Galactosidase Antibody in Western Blot (WB)
          beta Galactosidase Antibody in ELISA (ELISA)

          Product Details

          MA1-152

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          View 2 publications 2 publications

          ELISA (ELISA)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bacteria

          Published species

          Bacteria, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          DC1 4C7

          Immunogen

          Full length E. coli beta Galactosidase

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536860

          Product Specific Information

          Western blot analysis of MA1-152 detects recombinant E. coli beta Galactosidase at ~110 kDa. This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells.

          Target Information

          Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Beta-Gal; betagal; bgal; β-gal; β-galactosidase; βgal

          View more View less

          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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