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Invitrogen
ARF6 Monoclonal Antibody (6ARF01)
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FIGURE: 1 / 4
ARF6 Antibody (MA5-11795) in ICC/IF
Immunofluorescent analysis of ADP-ribosylation Factor 6 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an ADP-ribosylation Factor 6 monoclonal antibody (Product # MA5-11795) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed w... View More
Product Details
MA5-11795
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6ARF01
Immunogen
ARF6 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11795 targets ADP-ribosylation Factor 6 in WB and ICC/IF applications and shows reactivity with Human and Mouse samples.
The MA5-11795 immunogen is aRF6 protein.
Target Information
ARF6 (ADP-ribosylation factor 6) is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell-surface and intracellular membranes and whose functions may include tissue-specific effects on vesicular trafficking during cellular differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP ribosylation factor 6; ADP-ribosylation factor 6; ARF6; DKFZp564M0264; small GTP binding protein; unnamed protein product
Gene Aliases: AI788669; ARF6; AW496366
UniProt ID: (Human) P62330, (Mouse) P62331
Entrez Gene ID: (Human) 382, (Mouse) 11845
mitotic cytokinesis
liver development
intracellular protein transport
vesicle docking involved in exocytosis
cell adhesion
nervous system development
regulation of neuron projection development
positive regulation of neuron projection development
protein transport
vesicle-mediated transport
cell differentiation
positive regulation of actin filament polymerization
cortical actin cytoskeleton organization
endocytic recycling
protein localization to cell surface
regulation of Rac protein signal transduction
protein localization to endosome
negative regulation of receptor-mediated endocytosis
synaptic vesicle endocytosis
positive regulation of protein secretion
cell division
regulation of filopodium assembly
positive regulation of keratinocyte migration
regulation of dendritic spine development
cellular response to diacyl bacterial lipopeptide
protein localization to plasma membrane
establishment of epithelial cell polarity
ruffle assembly
hepatocyte apoptotic process
maintenance of postsynaptic density structure
positive regulation of focal adhesion disassembly
erythrocyte apoptotic process
positive regulation of protein localization to plasma membrane
positive regulation of mitotic cytokinetic process
protein localization to cleavage furrow
regulation of presynapse assembly
cellular response to nerve growth factor stimulus
negative regulation of protein localization to cell surface
negative regulation of dendrite development
transport
cell cycle
small GTPase mediated signal transduction
ruffle organization
myeloid cell apoptotic process
regulation of toll-like receptor 4 signaling pathway
positive regulation of establishment of protein localization to plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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