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Invitrogen
CD25 (Basiliximab Biosimilar) Recombinant Rabbit Monoclonal Antibody (Basiliximab)
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FIGURE: 1 / 3
CD25 (Basiliximab Biosimilar) Antibody (MA5-47836) in Flow
Flow Cytometry analysis of Basiliximab in Cynomolgus monkey lymphocytes using CD25 (Basiliximab Biosimilar) Monoclonal Antibody (Product # MA5-47836). Cells were stained with an isotype control, FITC Chimeric monoclonal antibody (Product # MA5-47752) (panel A), or CD25 (Basiliximab Biosimilar) Monoclonal Antibody (panel B) at a concentration of 1 µg/mL for 30 mins at RT. After washing, bound antibody was de... View More
Product Details
MA5-47836
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human,
Rhesus monkey
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Basiliximab
Immunogen
Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Specificity: Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit-binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (alpha-Beta-gamma-c)-T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
MA5-47836 is a chimeric antibody. The variable regions from one species have been grafted to the backbone of the other species.
Target Information
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD25; CD25; rhIL-2RA; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor precursor; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; TAC antigen; unnamed protein product
Gene Aliases: CD25; IDDM10; IL-2RA; IL2R; IL2RA; IMD41; p55; TCGFR
UniProt ID: (Rhesus monkey) Q5MNY4, (Human) P01589
Entrez Gene ID: (Rhesus monkey) 574300, (Human) 3559, (Cynomolgus monkey) 102123605
immune system process
inflammatory response to antigenic stimulus
regulation of T cell tolerance induction
regulation of immune system process
apoptotic process
activation-induced cell death of T cells
inflammatory response
immune response
cell surface receptor signaling pathway
Notch signaling pathway
interleukin-2-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
negative regulation of T cell proliferation
T cell homeostasis
positive regulation of T cell differentiation
regulation of T cell homeostatic proliferation
lymphocyte proliferation
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
activated T cell proliferation
regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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