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Invitrogen
RAR gamma-1 Polyclonal Antibody
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FIGURE: 1 / 4
RAR gamma-1 Antibody (PA3-813) in WB
Western blot was performed using Anti-RAR gamma-1 Polyclonal Antibody (Product # PA3-813) and a 60 kDa band corresponding to RAR gamma-1 was observed in MCF-7, HaCaT and Mouse Mammary Gland but not in BeWo and HepG2. Nuclear enriched extracts (50 µg lysate) of MCF7 (Lane 1), BeWo (Lane 2), HaCaT (Lane 3), Hep G2 (Lane 4) and tissue extract (50 µg lysate) of Mouse Mammary gland (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (P... View More
Product Details
PA3-813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues E(40) M L S P S F R G L G Q P D L P K E(57) of the N-terminal end of RAR gamma-1.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-813 detects recombinant human retinoic acid receptor (RAR) gamma-1. The immunizing sequence is 100% conserved in mouse. This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.
PA3-813 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RAR gamma-1 from transiently transfected 293T cells. PA3-813 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.
PA3-813 immunizing peptide corresponds to the N-terminus of RAR gamma-1. This peptide (Cat. # PEP-228) is available for use in neutralization and control experiments.
Target Information
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD. The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor subfamily 1 group B member 3; RAR gamma 2; RAR Gamma-1; RAR-gamma; retinoic acid; retinoic acid receptor; Retinoic acid receptor gamma; retinoic acid receptor gamma 2; retinoic acid receptor gamma 4; unnamed protein product
Gene Aliases: NR1B3; RAR-gamma; RARC; RARD; RARG; RARgamma; RARgamma2
UniProt ID: (Human) P13631, (Mouse) P18911
Entrez Gene ID: (Human) 5916, (Mouse) 19411, (Rat) 685072
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
retinoid X receptor binding
sequence-specific double-stranded DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
steroid hormone receptor activity
retinoic acid receptor activity
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
neural tube closure
chondrocyte development
glandular epithelial cell development
growth plate cartilage development
growth plate cartilage chondrocyte growth
regulation of transcription, DNA-templated
apoptotic process
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell size
anterior/posterior pattern specification
regulation of gene expression
positive regulation of gene expression
programmed cell death
cell differentiation
embryonic camera-type eye development
regulation of myelination
negative regulation of chondrocyte differentiation
response to retinoic acid
embryonic hindlimb morphogenesis
multicellular organism growth
positive regulation of apoptotic process
positive regulation of programmed cell death
negative regulation of cell differentiation
regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
embryonic eye morphogenesis
retinoic acid receptor signaling pathway
reproductive structure development
gland development
canonical Wnt signaling pathway
limb development
face development
bone development
bone morphogenesis
epithelium development
trachea cartilage development
prostate gland epithelium morphogenesis
negative regulation of cartilage development
Harderian gland development
cellular response to retinoic acid
stem cell proliferation
cellular response to leukemia inhibitory factor
negative regulation of stem cell proliferation
retinal pigment epithelium development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
camera-type eye development
negative regulation of apoptotic process
steroid hormone mediated signaling pathway
retina development in camera-type eye
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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