Search
Invitrogen
PABP Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 15
PABP Antibody (PA5-29883) in ICC/IF
Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Rabbit Polyclonal Antibody(Product # PA5-29883) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
Product Details
PA5-29883
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PABP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.79 mg/mL
Amount
79 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: PABP 1; PABP-1; poly A binding protein 1; poly(A) binding protein, cytoplasmic 2; poly(A)-binding protein 1; Polyadenylate-binding protein 1; unnamed protein product
Gene Aliases: ePAB; PAB1; PABP; PABP1; PABPC1; PABPC2; PabpI; PABPL1
UniProt ID: (Human) P11940, (Rat) Q9EPH8, (Mouse) P29341
Entrez Gene ID: (Human) 26986, (Rat) 171350, (Mouse) 18458
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA processing
RNA splicing
gene silencing by RNA
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA splicing, via spliceosome
positive regulation of viral genome replication
mRNA stabilization
CRD-mediated mRNA stabilization
negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
positive regulation of cytoplasmic translation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support