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Invitrogen

WT1 Polyclonal Antibody

View all (72) WT1 antibodies

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Product Details

PA5-85096

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wilms Tumor 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Amount

109 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792244

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (97%), Rat (97%), Japanese Medaka (83%), Xenopus laevis (87%), Pig (97%), Chicken (94%).

Positive Control: Mouse testis, Rat testis.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ewing sarcoma-Wilms tumor gene fusion; unnamed protein product; Wilms tumor 1; Wilms tumor protein; Wilms tumor protein homolog; Wilms' tumor protein; WT; WT33

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Gene Aliases: AWT1; D630046I19Rik; GUD; NPHS4; WAGR; WIT-2; WT-1; WT1; WT33

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UniProt ID: (Human) P19544, (Rat) P49952

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Entrez Gene ID: (Human) 7490, (Mouse) 22431, (Rat) 24883

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding zinc ion binding double-stranded methylated DNA binding sequence-specific DNA binding hemi-methylated DNA-binding metal ion binding C2H2 zinc finger domain binding sequence-specific double-stranded DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding double-stranded DNA binding transcription regulatory region DNA binding C2H2 zinc finger transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter vasculogenesis ureteric bud development branching involved in ureteric bud morphogenesis kidney development regulation of organ formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter germ cell development thorax and anterior abdomen determination heart development sex determination negative regulation of cell proliferation RNA splicing gonad development male gonad development tissue development positive regulation of gene expression negative regulation of translation negative regulation of cell growth adrenal gland development male genitalia development epithelial cell differentiation glomerulus development glomerular basement membrane development adrenal cortex formation camera-type eye development positive regulation of apoptotic process negative regulation of apoptotic process hypermethylation of CpG island negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mesenchymal to epithelial transition positive regulation of heart growth diaphragm development cardiac muscle cell fate commitment visceral serous pericardium development cellular response to cAMP cellular response to gonadotropin stimulus metanephric mesenchyme development nephron tubule morphogenesis glomerular visceral epithelial cell differentiation posterior mesonephric tubule development metanephric epithelium development metanephric S-shaped body morphogenesis negative regulation of metanephric glomerular mesangial cell proliferation circulatory system development positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of male gonad development negative regulation of female gonad development positive regulation of metanephric ureteric bud development metanephros development mesonephros development transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process spermatogenesis single fertilization regulation of gene expression sperm motility Sertoli cell development coronary vasculature development seminiferous tubule development negative regulation of mesenchymal cell apoptotic process involved in metanephros development
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