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Invitrogen
COL1A1 Polyclonal Antibody
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FIGURE: 1 / 13
COL1A1 Antibody (PA5-95137) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Collagen I in paraffin-embedded section of human lung adenocarcinoma cancer tissue using Collagen I Polyclonal Antibody (Product # PA5-95137). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µ... View More
Product Details
PA5-95137
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, rat skin tissue, rat lung tissue, mouse skin tissue, mouse NIH/3T3 whole cell. IHC: human liver cancer tissue, human lung adenocarcinoma cancer tissue, human renal cell carcinoma tissue, human bladder urothelial carcinoma tissue, human placenta tissue, mouse lung tissue, rat lung tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is the pro-alpha1 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 1 (I) chain propeptide; alpha-1 collagen type 1; alpha-1 collagen type I; alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; COL1A1; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen Col1-ColIII-1; collagen Col1-ColIII-2; Collagen I; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; Collagen1A1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen; unnamed protein product
Gene Aliases: CAFYD; Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSARTH1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4
UniProt ID: (Human) P02452, (Rat) P02454, (Mouse) P11087
Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842
skeletal system development
ossification
blood vessel development
osteoblast differentiation
intramembranous ossification
endochondral ossification
response to nutrient
visual perception
sensory perception of sound
response to xenobiotic stimulus
response to mechanical stimulus
positive regulation of epithelial to mesenchymal transition
negative regulation of cell-substrate adhesion
protein transport
collagen fibril organization
positive regulation of cell migration
response to nutrient levels
response to estradiol
response to insulin
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
collagen-activated tyrosine kinase receptor signaling pathway
response to hydrogen peroxide
response to peptide hormone
skin development
skin morphogenesis
cellular response to fibroblast growth factor stimulus
positive regulation of transcription, DNA-templated
animal organ development
response to steroid hormone
skeletal system morphogenesis
embryonic skeletal system development
system development
response to cAMP
response to hyperoxia
face morphogenesis
bone trabecula formation
cartilage development involved in endochondral bone morphogenesis
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to glucose stimulus
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
response to fluoride
cellular response to fluoride
response to corticosteroid
wound healing
response to drug
tooth eruption
protein heterotrimerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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