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          • Primary Antibodies ›
          • RPS27A Antibodies

          Invitrogen

          RPS27A Polyclonal Antibody

          View all (10) RPS27A antibodies

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          Cite RPS27A Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          RPS27A Antibody in Immunocytochemistry (ICC/IF)
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          RPS27A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          RPS27A Antibody (PA5-96039) in ICC/IF

          Immunofluorescence analysis of RPS27A in MCF7 cells. Samples were incubated with RPS27A Polyclonal antibody (Product # PA5-96039) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RPS27A Antibody in Immunocytochemistry (ICC/IF)
          RPS27A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPS27A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPS27A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          RPS27A Antibody in Western Blot (WB)

          Product Details

          PA5-96039

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein (or fragment)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.98 mg/mL

          Amount

          98 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807841

          Product Specific Information

          Positive Samples: Jurkat, NIH/3T3, Mouse kidney, Mouse liver, Rat brain; Cellular Location: Cytoplasm, Nucleus

          Target Information

          Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 40S ribosomal protein S27a; epididymis luminal protein 112; pro-ubiquitin; ubiquitin and ribosomal protein S27a; ubiquitin C; Ubiquitin carboxyl extension protein 80; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80; Ubiquitin-ribosomal protein eS31 fusion protein; unnamed protein product

          View more View less

          Gene Aliases: 0610006J14Rik; CEP80; eS31; HEL112; RPS27A; S27A; Uba52; UBA80; Ubb; UBC; UBCEP1; UBCEP80

          View more View less

          UniProt ID: (Rat) P62982, (Human) P62979, (Mouse) P62983

          View more View less

          Entrez Gene ID: (Rat) 100912032, (Human) 6233, (Mouse) 78294

          View more View less

          Function(s)
          RNA binding structural constituent of ribosome protein binding zinc ion binding protein tag ubiquitin protein ligase binding metal ion binding poly(A) RNA binding
          Process(es)
          cytoplasmic translation translation protein ubiquitination modification-dependent protein catabolic process ribosomal small subunit biogenesis biological_process
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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