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Invitrogen
Equine IL-8 Recombinant Protein
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Product Details
REIL8I
Product Specifications
Class
Recombinant
Type
Protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
20 µg
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant equine IL-8 was determined by its ability to induce equine neutrophil migration in vitro. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically 22 ng/mL.
Expression: Mature equine IL-8 was expressed in Pichia pastoris, X-33. GenBank® Accession # AAO37764,
amino acids 23-101. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: *ITAELRCQCI. *The first five amino acids of the expressed protein appear to be cleaved from the N-terminus.
Reconstitute with 0.5 mL of distilled water.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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