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Invitrogen
HIV1 Reverse Transcriptase Recombinant Protein
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FIGURE: 1 / 2
HIV1 Reverse Transcriptase Protein (RP-4912) in WB Ctrl
Western blot analysis of functional recombinant full-length HIV-1 reverse transcriptase (Product # RP-4912). Anti-HIV-1 RT antibody was used at 1:2,000 dilution. A secondary, goat anti-rabbit IgG antibody conjugated with HRP was used at 1:5,000 dilution. An ECL system was used for detection. Protein was run at the following concentrations: 40 ng/ lane, 20 ng/ lane, 4 ng/ lane, and 2 ng/ lane.
Product Details
RP-4912
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
5000 U/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
RP-4912 can be used as a positive control in Western blotting with ABR antibodies againt -HIV-1 Reverse Transcriptase, as well as in screening of specific inhibitors for new AIDS treatment .
RP-4912 is composed of two subunits with calculated molecular weights of ~66 kDa and ~51 kDa; see figure 1.
RP-4912 has a specific activity of 5,000 units/mL
Target Information
HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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