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Invitrogen
Human BCLAF1 (aa 646-764) Control Fragment Recombinant Protein
Product Details
RP-89287
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
QKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSR
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-55686. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
BCLAF1 is an intracellular, death promoting transcriptional repressor of survival genes that interacts with several members of the BCL2 family of proteins. It shows homology to the basic leucine zipper (bzip) and MYB DNA binding domains.From recent research it can be concluded that in case of apoptotic cells this protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope. When over expressed this protein induces apoptosis and can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal, potentially helpful to overcome the resistance to STI571.BCLAF1 is found to express mostly in skeletal muscles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2-associated transcription factor 1; BCL2-associated transcription factor 1; BCLAF1 and THRAP3 family member 1; BP-1; Btf; Btf-s
Gene Aliases: BCLAF1; bK211L9.1; BTF; KIAA0164
UniProt ID: (Human) Q9NYF8
Entrez Gene ID: (Human) 9774
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