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Human BD-3 Recombinant Protein, PeproTech®

Human BD-3 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human BD-3 Protein (300-52-100UG) in Functional

Bioassay analysis of Human BD-3 Recombinant Protein, PeproTech® (Product # 300-52-1MG).

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Product Details

300-52-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse, Virus

Expression System

E. coli

Amino acid sequence

GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK

Molecular weight

5.1 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.^M
^M
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Oct 2014].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hybrid derivative of cathelicidin and human beta defensin-2 against Gram-positive bacteria: A novel approach for the treatment of bacterial keratitis.

Scientific reports

Ting DSJ,Goh ETL,Mayandi V,Busoy JMF,Aung TT,Periayah MH,Nubile M,Mastropasqua L,Said DG,Htoon HM,Barathi VA,Beuerman RW,Lakshminarayanan R,Mohammed I,Dua HS

Wed Sep 15 00:00:00 UTC 2021

HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines.

Antibiotics (Basel, Switzerland)

Gomez Hernandez MP,Bates AM,Starman EE,Lanzel EA,Comnick C,Xie XJ,Brogden KA

300-52 was used in In vitro experiments to investigate human β-defensin 3 in head and neck squamous cell carcinoma and oral squamous cell carcinoma.

Tue Sep 24 00:00:00 UTC 2019

pH modulates the activity and synergism of the airway surface liquid antimicrobials β-defensin-3 and LL-37.

Proceedings of the National Academy of Sciences of the United States of America

Abou Alaiwa MH,Reznikov LR,Gansemer ND,Sheets KA,Horswill AR,Stoltz DA,Zabner J,Welsh MJ

Tue Dec 30 00:00:00 UTC 2014

Human β-defensin HBD3 binds to immobilized Bla g2 from the German cockroach (Blattella germanica).

Peptides

Dietrich DE,Martin AD,Brogden KA

Sat Mar 01 00:00:00 UTC 2014

Histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and alters HagB-induced chemokine responses.

Scientific reports

Borgwardt DS,Martin AD,Van Hemert JR,Yang J,Fischer CL,Recker EN,Nair PR,Vidva R,Chandrashekaraiah S,Progulske-Fox A,Drake D,Cavanaugh JE,Vali S,Zhang Y,Brogden KA

Wed Jan 29 00:00:00 UTC 2014

Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.

Scientific reports

Harvey LE,Kohlgraf KG,Mehalick LA,Raina M,Recker EN,Radhakrishnan S,Prasad SA,Vidva R,Progulske-Fox A,Cavanaugh JE,Vali S,Brogden KA

Fri Aug 16 00:00:00 UTC 2013

Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.

Scientific reports

Harvey LE,Kohlgraf KG,Mehalick LA,Raina M,Recker EN,Radhakrishnan S,Prasad SA,Vidva R,Progulske-Fox A,Cavanaugh JE,Vali S,Brogden KA

Fri Aug 16 00:00:00 UTC 2013

Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.

Scientific reports

Harvey LE,Kohlgraf KG,Mehalick LA,Raina M,Recker EN,Radhakrishnan S,Prasad SA,Vidva R,Progulske-Fox A,Cavanaugh JE,Vali S,Brogden KA

Fri Aug 16 00:00:00 UTC 2013

SapF-mediated heme-iron utilization enhances persistence and coordinates biofilm architecture of Haemophilus.

Frontiers in cellular and infection microbiology

Vogel AR,Szelestey BR,Raffel FK,Sharpe SW,Gearinger RL,Justice SS,Mason KM

Wed Dec 11 00:00:00 UTC 2013

An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.

PloS one

Jin G,Kawsar HI,Hirsch SA,Zeng C,Jia X,Feng Z,Ghosh SK,Zheng QY,Zhou A,McIntyre TM,Weinberg A

Tue Jun 08 00:00:00 UTC 2010

Streptococcus agalactiae CspA is a serine protease that inactivates chemokines.

Journal of bacteriology

Bryan JD,Shelver DW

Sun Mar 01 00:00:00 UTC 2009

Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.

BMC oral health

Kalus AA,Fredericks LP,Hacker BM,Dommisch H,Presland RB,Kimball JR,Dale BA

Thu Aug 27 00:00:00 UTC 2009

Human beta-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response.

Immunology and cell biology

Pingel LC,Kohlgraf KG,Hansen CJ,Eastman CG,Dietrich DE,Burnell KK,Srikantha RN,Xiao X,Bélanger M,Progulske-Fox A,Cavanaugh JE,Guthmiller JM,Johnson GK,Joly S,Kurago ZB,Dawson DV,Brogden KA

Mon Jun 01 00:00:00 UTC 2009

Haemophilus ducreyi is resistant to human antimicrobial peptides.

Antimicrobial agents and chemotherapy

Mount KL,Townsend CA,Bauer ME

Sat Sep 01 00:00:00 UTC 2007

Injury-induced innate immune response in human skin mediated by transactivation of the epidermal growth factor receptor.

The Journal of clinical investigation

Sørensen OE,Thapa DR,Roupé KM,Valore EV,Sjöbring U,Roberts AA,Schmidtchen A,Ganz T

Sat Jul 01 00:00:00 UTC 2006

TGF-alpha regulates TLR expression and function on epidermal keratinocytes.

Journal of immunology (Baltimore, Md. : 1950)

Miller LS,Sørensen OE,Liu PT,Jalian HR,Eshtiaghpour D,Behmanesh BE,Chung W,Starner TD,Kim J,Sieling PA,Ganz T,Modlin RL

Sun May 15 00:00:00 UTC 2005

Expression and activity of beta-defensins and LL-37 in the developing human lung.

Journal of immunology (Baltimore, Md. : 1950)

Starner TD,Agerberth B,Gudmundsson GH,McCray PB

Tue Feb 01 00:00:00 UTC 2005

Attribution of the various inhibitory actions of the streptococcal inhibitor of complement (SIC) to regions within the molecule.

The Journal of biological chemistry

Binks MJ,Fernie-King BA,Seilly DJ,Lachmann PJ,Sriprakash KS

Fri May 20 00:00:00 UTC 2005

Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms.

Journal of clinical microbiology

Joly S,Maze C,McCray PB,Guthmiller JM

Mon Mar 01 00:00:00 UTC 2004

Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors.

Journal of immunology (Baltimore, Md. : 1950)

Sørensen OE,Cowland JB,Theilgaard-Mönch K,Liu L,Ganz T,Borregaard N

Sun Jun 01 00:00:00 UTC 2003

Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation.

Infection and immunity

Starner TD,Swords WE,Apicella MA,McCray PB

Sun Sep 01 00:00:00 UTC 2002

The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus.

The Journal of biological chemistry

Schibli DJ,Hunter HN,Aseyev V,Starner TD,Wiencek JM,McCray PB,Tack BF,Vogel HJ

Fri Mar 08 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: BD-3; beta defensin 3; beta defensin-3; Beta-defensin 103; Beta-defensin 3; Defensin, beta 103; defensin, beta 103A; defensin, beta 3; Defensin-like protein

View more View less

Gene Aliases: BD-3; BD3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; hBD-3; HBD3; HBP-3; HBP3

Human BD-3 Recombinant Protein, PeproTech®

Human BD-3 Protein (300-52-100UG) in Functional

Product Details

300-52-100UG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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