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Invitrogen
Human CDK2/cyclin E1, GST Tag Recombinant Protein
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Product Details
PV6295
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
1-298
Tag
GST-tag
Molecular weight
60.2-73.4 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 10mM glutathione, 150mM NaCl, 0.1mM PMSF, 0.25mM DTT, 0.1mM EDTA, 25% glycerol
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
The gene accession number for the subunits CKD2 and Cyclin E1 are NP_001789.2 and NP_001229.1.
Target Information
CDK2, or Cyclin-Dependent Kinase 2, is a critical member of the cyclin-dependent kinase family, which regulates cell cycle progression. CDK2 is particularly important for the transition from the G1 phase to the S phase and for the progression through the S phase of the cell cycle.
Cyclin E is a regulatory subunit that binds to CDK2 and activates its kinase activity. The binding of Cyclin E to CDK2 is necessary for the proper functioning of CDK2, as cyclins regulate the activation and specificity of CDKs.
Together, the CDK2/Cyclin E complex plays a pivotal role in cell cycle regulation by phosphorylating key substrates, including the retinoblastoma protein (Rb), which leads to the activation of E2F transcription factors and the initiation of DNA replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cdc2-related protein kinase; Cell division protein kinase 2; Cyclin-dependent kinase 2; G1/S-specific cyclin-E1; p33 protein kinase
Gene Aliases: CCNE; CCNE1; CDK2; CDKN2; p33(CDK2); pCCNE1
UniProt ID: (Human) P24864, (Human) P24941
Entrez Gene ID: (Human) 898, (Human) 1017
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