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Invitrogen
Human CDK4 Recombinant Protein
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Product Details
RP-75531
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Amino acids Met1- Glu303 containing an N-terminal His-tag
Tag
His-tag
Molecular weight
37.47 kDa
Class
Recombinant
Type
Protein
Purity
>95%, by SDS-PAGE under reducing conditions
Conjugate
Form
Liquid
Concentration
0.081 mg/mL
Purification
purified
Storage buffer
0.01M Bis-tris propane, pH 9.0, with 0.1M NaCl, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
This product contains purified recombinant human CDK4 protein with an N-terminal hexahistidine tag.
This product may be used as a positive control in many immunological assays.
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 4; Cyclin-dependent kinase 4; MGC14458; PSK-J3
Gene Aliases: CDK4; CMM3; PSK-J3
UniProt ID: (Human) P11802
Entrez Gene ID: (Human) 1019
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