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Invitrogen
Human CEACAM5 (CD66e) Recombinant Protein
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Product Details
A42584
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human CEACAM5, amino acids Met1-Ala685 (Accession # NP_004354.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
72.8 kDa
Class
Recombinant
Type
Protein
Purity
≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1.Loaded Anti-Human CEACAM5 antibody, human IgG1 on ProA Biosensor, can bind Human CEACAM5 (CD66e) Recombinant Protein with an affinity constant of 3.72 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
2.Immobilized Anti-CEA Antibody at 2 µg/mL (100 µL/well) can bind Human CEACAM5 (CD66e) Recombinant Protein, The EC50 is 5.5-16 ng/mL.
2.Immobilized Anti-CEA Antibody at 2 µg/mL (100 µL/well) can bind Human CEACAM5 (CD66e) Recombinant Protein, The EC50 is 5.5-16 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM tris, pH 8, with 5% mannitol, 5% trehalose, 150mM NaCl, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CEA (Carcino Embryonic Antigen, CD66e) is synthesized during development in the fetal gut, and re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA is a member of a large family of glycoproteins, a useful tumor marker for adenocarcinoma, and found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. CEA was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, CEA was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; CEA cell adhesion molecule 5; Cell adhesion molecule CEACAM5; Meconium antigen 100; OTTHUMP00000199033; sCD66e; soluble CD66e
Gene Aliases: CD66e; CEA; CEACAM5
UniProt ID: (Human) P06731
Entrez Gene ID: (Human) 1048
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