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Product Details
RP-100963
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AIITQVTNPKEDEGRLPGAGEKASQCNVSLKKQRSRSILSSFFCCFRDYNVEAPPPSSPSVLPPLVEENGGLQKGDQ
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61713. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like; CTD small phosphatase-like protein; CTDSP-like; hYA22; MGC71888; NIF-like protein; NLI-interacting factor 1; Nuclear LIM interactor-interacting factor 1; Protein YA22; RB protein serine phosphatase from chromosome 3; RBSP3; SCP3; SCP3 COR1; Small C-terminal domain phosphatase 3; small CTD phosphatase 3
Gene Aliases: C3orf8; CTDSPL; HYA22; NIF1; NIFL; PSR1; RBSP3; SCP3; YA22
UniProt ID: (Human) O15194
Entrez Gene ID: (Human) 10217
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