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Invitrogen
Human GTF2F2 (aa 153-247) Control Fragment Recombinant Protein
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Product Details
RP-89428
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
YKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (97%), Rat (97%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82267. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
GTF2F2 is required for both basal and HIV-1 Tat-activated transcription. GTF2F2 synergizes with HIV-1 Tat and the cellular co-activator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; General transcription factor IIF subunit 2; general transcription factor IIF, polypeptide 2, 30kDa; TFIIF beta; TFIIF-beta; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30
Gene Aliases: BTF4; GTF2F2; RAP30; TF2F2; TFIIF
UniProt ID: (Human) P13984
Entrez Gene ID: (Human) 2963
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