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Invitrogen
Human IL-12 p70 Recombinant Protein
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Product Details
14-8129-62
Product Specifications
Expression System
Insect cells
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
5 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-gamma) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.
Applications Reported: Recombinant human IL-12 is biologically active and has been reported for use in ELISA and bioassay.
Applications Tested: This recombinant human IL-12 p70 has been tested by sandwich ELISA using the human IL-12 p70 ELISA (B-T21/C8.6). This IL-12 p70 protein has been tested by bioassay, yielding an ED50 of 100 pg/mL, corresponding to a specific activity of approximately 1x10e7 Units/mg.
Source: Insect cells infected with baculovirus: human p40, amino acids met 1-Ser 328, accession # NM_002187 was co-expressed with human p35, amino acids met 1-Ser-253, accession # NM_000882.
Bioactivity: Measured by dose-dependent induction of proliferation of PHA-activated human peripheral blood mononuclear cells. The ED50 is 100 pg/mL, corresponding to a specific activity of 1.0 x 10e7 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The heterodimeric, p40, amino acids ile 23-ser 328, cystine linked to p35, amino acids arg 57-ser 253, has a predicted molecular mass of 57,239. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 67 kDa protein. The DTT reduced protein migrates as 43 kDa and 25 kDa polypeptides.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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