Human IL-2 Recombinant Protein

Product Specific Information

Description: Human IL-2 (also known as TCGF) is a ~15 kDa factor produced mainly by activated CD4^+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

Applications Reported: Recombinant human IL-2 is biologically active and can promote proliferation of T lymphocytes in culture. It is also used as standard in human IL-2 ELISA.

Applications Tested: The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Source: E. coli-expressed amino acids Ala 21-Thr 153, accession #NM_000586.

Bioactivity: The ED50 of this protein, as measured by CTLL-2 proliferation assay, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.