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Invitrogen
Human LAG-3 (CD223) Fc Chimera Recombinant Protein
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Product Details
RP-88095
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human LAG3, amino acids Met1-Leu450 (Accession # NP_002277.4) with a C-terminal human IgG1 Fc region
Tag
Fc-tag
Molecular weight
84.19 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized LAG3 Protein, Human, Recombinant (ECD, hFc Tag) at 2 µg/mL (100 µL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL.
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
purified
Storage buffer
0.1M Glutamine/20mM sodium phosphate, pH 7.4, with 0.1M arginine, 10% glycerol, 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 20, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3
Gene Aliases: CD223; FDC; LAG3
UniProt ID: (Human) P18627
Entrez Gene ID: (Human) 3902
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