Gibco

Human Leptin Recombinant Protein, PeproTech®

Product Details

300-27-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 13 publications 13 publications

Product Specifications

Species

Human

Published species

Bacteria, Human, Mouse, Non-human primate, Pig, Rat

Expression System

E. coli

Amino acid sequence

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC

Molecular weight

16 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

PeproTech's Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 ug Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Leptin is a 16.0 kDa protein containing 147 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer.

Cancer discovery

Fonteneau G,Redding A,Hoag-Lee H,Sim ES,Heinrich S,Gaida MM,Grabocka E

300-27 was used in In vitro experiments to show that stress adaptation via the phase-separation organelles stress granules (SGs) mediates pancreatic ductal adenocarcinoma (PDAC) development.

Fri Aug 05 00:00:00 UTC 2022

Cutting Edge: Intestinal IL-17A Receptor Signaling Specifically Regulates High-Fat Diet-Mediated, Microbiota-Driven Metabolic Disorders.

Journal of immunology (Baltimore, Md. : 1950)

Gaudino SJ,Huang H,Jean-Pierre M,Joshi P,Beaupre M,Kempen C,Wong HT,Kumar P

Fri Oct 15 00:00:00 UTC 2021

The metabolic hormone leptin promotes the function of T_FH cells and supports vaccine responses.

Nature communications

Deng J,Chen Q,Chen Z,Liang K,Gao X,Wang X,Makota FV,Ong HS,Wan Y,Luo K,Gong D,Yu X,Camuglia S,Zeng Q,Zhou T,Xue F,He J,Wei Y,Xiao F,Ma J,Hill DL,Pierson W,Nguyen THO,Zhou H,Wang Y,Shen W,Sun L,Li Z,Xia Q,Qian K,Ye L,Rockman S,Linterman MA,Kedzierska K,Shen N,Lu L,Yu D

Mon May 24 00:00:00 UTC 2021

Generation of hypothalamic arcuate organoids from human induced pluripotent stem cells.

Cell stem cell

Huang WK,Wong SZH,Pather SR,Nguyen PTT,Zhang F,Zhang DY,Zhang Z,Lu L,Fang W,Chen L,Fernandes A,Su Y,Song H,Ming GL

300-27 was used in Cell Culture to report the generation of ARCOs from human iPSCs to model the development of the human hypothalamic arcuate nucleus.

Thu Sep 02 00:00:00 UTC 2021

Leptin-mediated differential regulation of microsomal triglyceride transfer protein in the intestine and liver affects plasma lipids.

The Journal of biological chemistry

Iqbal J,Mascareno E,Chua S,Hussain MM

Fri Mar 27 00:00:00 UTC 2020

Preclinical Assessment of Leptin Transport into the Cerebrospinal Fluid in Diet-Induced Obese Minipigs.

Obesity (Silver Spring, Md.)

Chmielewski A,Hubert T,Descamps A,Mazur D,Daoudi M,Ciofi P,Fontaine C,Caiazzo R,Pattou F,Prevot V,Pigeyre M

Sat Jun 01 00:00:00 UTC 2019

Leptin induces TNFα-dependent inflammation in acquired generalized lipodystrophy and combined Crohn's disease.

Nature communications

Ziegler JF,Böttcher C,Letizia M,Yerinde C,Wu H,Freise I,Rodriguez-Sillke Y,Stoyanova AK,Kreis ME,Asbach P,Kunkel D,Priller J,Anagnostopoulos I,Kühl AA,Miehle K,Stumvoll M,Tran F,Fredrich B,Forster M,Franke A,Bojarski C,Glauben R,Löscher BS,Siegmund B,Weidinger C

Tue Dec 10 00:00:00 UTC 2019

Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.

Cell stem cell

Rajamani U,Gross AR,Hjelm BE,Sequeira A,Vawter MP,Tang J,Gangalapudi V,Wang Y,Andres AM,Gottlieb RA,Sareen D

300-27 was used in Cell Culture to investigate hypothalamic neurons in obesity via human induced pluripotent stem cells.

Thu May 03 00:00:00 UTC 2018

9c11tCLA modulates 11t18:1 and 9t18:1 induced inflammations differently in human umbilical vein endothelial cells.

Scientific reports

Li J,Hu SB,He YM,Zhuo CF,Zhou RL,Chen F,Li HY,Deng ZY

Wed Jan 24 00:00:00 UTC 2018

Differentiation of hypothalamic-like neurons from human pluripotent stem cells.

The Journal of clinical investigation

Wang L,Meece K,Williams DJ,Lo KA,Zimmer M,Heinrich G,Martin Carli J,Leduc CA,Sun L,Zeltser LM,Freeby M,Goland R,Tsang SH,Wardlaw SL,Egli D,Leibel RL

Sun Feb 01 00:00:00 UTC 2015

Increased activation and cytokine secretion in B cells stimulated with leptin in aged humans.

Immunity & ageing : I & A

Gupta S,Agrawal S,Gollapudi S

Wed Jan 23 00:00:00 UTC 2013

The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion.

PloS one

Spinnler R,Gorski T,Stolz K,Schuster S,Garten A,Beck-Sickinger AG,Engelse MA,de Koning EJ,Körner A,Kiess W,Maedler K

Fri Aug 02 00:00:00 UTC 2013

The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion.

PloS one

Spinnler R,Gorski T,Stolz K,Schuster S,Garten A,Beck-Sickinger AG,Engelse MA,de Koning EJ,Körner A,Kiess W,Maedler K

Fri Aug 02 00:00:00 UTC 2013

Leptin activates human B cells to secrete TNF-α, IL-6, and IL-10 via JAK2/STAT3 and p38MAPK/ERK1/2 signaling pathway.

Journal of clinical immunology

Agrawal S,Gollapudi S,Su H,Gupta S

Wed Jun 01 00:00:00 UTC 2011

Vascular endothelial growth factor receptor-2 couples cyclo-oxygenase-2 with pro-angiogenic actions of leptin on human endothelial cells.

PloS one

Garonna E,Botham KM,Birdsey GM,Randi AM,Gonzalez-Perez RR,Wheeler-Jones CP

Mon Apr 18 00:00:00 UTC 2011

Leptin increases L-type Ca2+ channel expression and GnRH-stimulated LH release in LbetaT2 gonadotropes.

Molecular and cellular endocrinology

Avelino-Cruz JE,Flores A,Cebada J,Mellon PL,Felix R,Monjaraz E

300-27 was used in Cell Culture to investigate the role of GnRH and leptin.

Tue Jan 27 00:00:00 UTC 2009

Effects of intranasal administration of a leptin-secreting Lactococcus lactis recombinant on food intake, body weight, and immune response of mice.

Applied and environmental microbiology

Bermúdez-Humarán LG,Nouaille S,Zilberfarb V,Corthier G,Gruss A,Langella P,Issad T

Wed Aug 01 00:00:00 UTC 2007

Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood.

Stem cells (Dayton, Ohio)

Chang YJ,Shih DT,Tseng CP,Hsieh TB,Lee DC,Hwang SM

Wed Mar 01 00:00:00 UTC 2006

Leptin enhances feeding suppression and neural activation produced by systemically administered bombesin.

American journal of physiology. Regulatory, integrative and comparative physiology

Ladenheim EE,Emond M,Moran TH

Mon Aug 01 00:00:00 UTC 2005

Insulin receptor substrate 2 plays a crucial role in beta cells and the hypothalamus.

The Journal of clinical investigation

Kubota N,Terauchi Y,Tobe K,Yano W,Suzuki R,Ueki K,Takamoto I,Satoh H,Maki T,Kubota T,Moroi M,Okada-Iwabu M,Ezaki O,Nagai R,Ueta Y,Kadowaki T,Noda T

Fri Oct 01 00:00:00 UTC 2004

Leptin modulates beta cell expression of IL-1 receptor antagonist and release of IL-1beta in human islets.

Proceedings of the National Academy of Sciences of the United States of America

Maedler K,Sergeev P,Ehses JA,Mathe Z,Bosco D,Berney T,Dayer JM,Reinecke M,Halban PA,Donath MY

Tue May 25 00:00:00 UTC 2004

Cytokine regulation of skeletal muscle fatty acid metabolism: effect of interleukin-6 and tumor necrosis factor-alpha.

American journal of physiology. Endocrinology and metabolism

Bruce CR,Dyck DJ

Fri Oct 01 00:00:00 UTC 2004

Evidence that paraventricular nucleus oxytocin neurons link hypothalamic leptin action to caudal brain stem nuclei controlling meal size.

American journal of physiology. Regulatory, integrative and comparative physiology

Blevins JE,Schwartz MW,Baskin DG

Thu Jul 01 00:00:00 UTC 2004

Feeding and temperature responses to intravenous leptin infusion are differential predictors of obesity in rats.

American journal of physiology. Regulatory, integrative and comparative physiology

Ruffin MP,Adage T,Kuipers F,Strubbe JH,Scheurink AJ,van Dijk G

Thu Apr 01 00:00:00 UTC 2004

Leptin induces the hepatic high density lipoprotein receptor scavenger receptor B type I (SR-BI) but not cholesterol 7alpha-hydroxylase (Cyp7a1) in leptin-deficient (ob/ob) mice.

The Journal of biological chemistry

Lundåsen T,Liao W,Angelin B,Rudling M

Fri Oct 31 00:00:00 UTC 2003

Activation of the leptin receptor by a ligand-induced conformational change of constitutive receptor dimers.

The Journal of biological chemistry

Couturier C,Jockers R

Fri Jul 18 00:00:00 UTC 2003

Activation of the leptin receptor by a ligand-induced conformational change of constitutive receptor dimers.

The Journal of biological chemistry

Couturier C,Jockers R

Fri Jul 18 00:00:00 UTC 2003

Interleukin-6-deficient mice develop mature-onset obesity.

Nature medicine

Wallenius V,Wallenius K,Ahrén B,Rudling M,Carlsten H,Dickson SL,Ohlsson C,Jansson JO

Tue Jan 01 00:00:00 UTC 2002

Elevated serum leptin in AEDS.

Allergy

Kimata H

Fri Feb 01 00:00:00 UTC 2002

Increased expression of the sterol regulatory element-binding protein-1 gene in insulin receptor substrate-2(-/-) mouse liver.

The Journal of biological chemistry

Tobe K,Suzuki R,Aoyama M,Yamauchi T,Kamon J,Kubota N,Terauchi Y,Matsui J,Akanuma Y,Kimura S,Tanaka J,Abe M,Ohsumi J,Nagai R,Kadowaki T

Fri Oct 19 00:00:00 UTC 2001

Placental leptin in normal, diabetic and fetal growth-retarded pregnancies.

Molecular human reproduction

Lea RG,Howe D,Hannah LT,Bonneau O,Hunter L,Hoggard N

Tue Aug 01 00:00:00 UTC 2000

NPY-leptin: opposing effects on appetitive and consummatory ingestive behavior and sexual behavior.

American journal of physiology. Regulatory, integrative and comparative physiology

Ammar AA,Sederholm F,Saito TR,Scheurink AJ,Johnson AE,Södersten P

Thu Jun 01 00:00:00 UTC 2000

Central leptin modulates behavioral and neural responsivity to CCK.

The American journal of physiology

Emond M,Schwartz GJ,Ladenheim EE,Moran TH

Sat May 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB-R; Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein

Human Leptin Recombinant Protein, PeproTech®

Product Details

300-27-1MG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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