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Thermo Scientific
Human MAP4K3 (GLK), GST Tag Recombinant Protein
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Product Details
PV6349
Product Specifications
Species
Human
Expression System
Baculovirus
Amino acid sequence
1-380
Tag
GST-tag
Molecular weight
69.6 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT, 25% glycerol
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots; aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
Recombinant human MAP4K3 (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
•Validated in LanthaScreen™ Kinase Binding Assay
•Protocols available for simple use and optimized performance
•Multiple pack sizes available for convenient use
Related Product:
LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant, Fragment
The gene accession number for MAP4K3 (GLK) is NP_003609.2.
Target Information
MAP4K3 (also known as GLK) is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. MAP4K3 is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-alpha and can lead to the activation of the JNK signaling pathway. MAP4K3 can directly activate PKC-T, during TCR signaling and people with systemic lupus erythematosus show considerable enhanced MAP4K3 expression and activation of PKC-T, and the kinase IKK in T cells. MAP4K3 can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase 1 (MEKK1). MAP4K3 previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase 3 (MAP4K3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: germinal center kinase-like kinase; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; Mitogen-activated protein kinase kinase kinase kinase 3
Gene Aliases: GLK; MAP4K3; MAPKKKK3; MEKKK 3; MEKKK3; RAB8IPL1
UniProt ID: (Human) Q8IVH8
Entrez Gene ID: (Human) 8491
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