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Invitrogen
Human NGF Recombinant Protein
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Product Details
A42578
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
CHO cells
Amino acid sequence
Human β-NGF, amino acids Ser122-Arg239 (Accession # NP_002497.2)
Molecular weight
13.2 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<1.0 EU/µg
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
40mM arginine/40mM histidine, pH 5.5, with 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B nerve growth factor; b-NGF; beta ngf; Beta-nerve growth factor; Beta-NGF; BetaNGF; bNGF; MGC161426; MGC161428; Nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGF b; NGF β; NGFbeta; NGFβ; β nerve growth factor; β NGF; βNGF
Gene Aliases: Beta-NGF; HSAN5; NGF; NGFB
UniProt ID: (Human) P01138
Entrez Gene ID: (Human) 4803
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