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Gibco
Human NT-4 Recombinant Protein
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Product Details
PHC7024
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human NT-4 recombinant protein is a homodimer containing two 130 amino acid subunits
Molecular weight
27.2 kDa
Class
Recombinant
Type
Protein
Purity
≥98% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 20 - 50 ng/mL; determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain septal cultures.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute rhNT-4 in sterile distilled water at a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: neurotrophic factor 4; neurotrophic factor 5; Neurotrophin 4/5; neurotrophin 5 (neurotrophin 4/5); Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT-4; NT-5; NT4/NT5
Gene Aliases: GLC10; GLC1O; NT-4; NT-4/5; NT-5; NT4; NT5; NTF4; NTF5
UniProt ID: (Human) P34130
Entrez Gene ID: (Human) 4909
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