Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Highest antigen sequence indentity to the following orthologs: Mouse (69%), Rat (69%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60672. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3' untranslated region of the mRNA to decode the UGA codon as Sec. SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins. Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA. SBP-2 shows binding activity in the liver and testis as well as hepatoma cells. SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1. A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells. SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements. SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp686C09169; OTTHUMP00000064929; OTTHUMP00000064930; OTTHUMP00000064931; OTTHUMP00000064932; RP11-89K14.1; SECIS-binding protein 2; Selenocysteine insertion sequence-binding protein 2
Gene Aliases: SBP2; SECISBP2
UniProt ID: (Human) Q96T21
Entrez Gene ID: (Human) 79048
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