Gibco

Human SPARC Recombinant Protein, PeproTech®

Product Details

120-36-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse, Rat

Expression System

CHO cells

Amino acid sequence

APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

Molecular weight

32.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation. The calculated molecular weight of Recombinant Human SPARC/Osteonectin is 32.7 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bidirectional epithelial-mesenchymal crosstalk provides self-sustaining profibrotic signals in pulmonary fibrosis.

The Journal of biological chemistry

Yao L,Zhou Y,Li J,Wickens L,Conforti F,Rattu A,Ibrahim FM,Alzetani A,Marshall BG,Fletcher SV,Hancock D,Wallis T,Downward J,Ewing RM,Richeldi L,Skipp P,Davies DE,Jones MG,Wang Y

Wed Sep 01 00:00:00 EDT 2021

SPARC promotes self-renewal of limbal epithelial stem cells and ocular surface restoration through JNK and p38-MAPK signaling pathways.

Stem cells (Dayton, Ohio)

Zhu J,Wang LY,Li CY,Wu JY,Zhang YT,Pang KP,Wei Y,Du LQ,Liu M,Wu XY

Wed Jan 01 00:00:00 EST 2020

Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.

Oncogene

John B,Naczki C,Patel C,Ghoneum A,Qasem S,Salih Z,Said N

Wed May 01 00:00:00 EDT 2019

A fragment of SPARC reflecting increased collagen affinity shows pathological relevance in lung cancer - implications of a new collagen chaperone function of SPARC.

Cancer biology & therapy

Kehlet SN,Manon-Jensen T,Sun S,Brix S,Leeming DJ,Karsdal MA,Willumsen N

120-36 was used in In vitro experiments to suggest that cleavage of SPARC at a specific site, which modulates collagen binding, is a physiological mechanism increased during pathogenesis of lung cancer.

Mon Sep 16 00:00:00 EDT 2019

Aging

Nakamura K,Nakano S,Miyoshi T,Yamanouchi K,Matsuwaki T,Nishihara M

Sun Jan 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC