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Invitrogen
Human TSP50 (aa 246-324) Control Fragment Recombinant Protein
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Product Details
RP-96485
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (65%), Rat (65%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83516. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Testes-specific protease 50 (TSP50) is a gene located within the chromosome region 3p21.31, primarily expressed in testicular tissue, and is homologous to serine proteases. However, TSP50 contains a distinctive catalytic triad where serine, a crucial catalytic residue, is replaced by threonine, allowing it to exhibit a novel protease activity. TSP50 is recognized for its role as a pro-oncogene, contributing significantly to cancer progression by promoting aerobic glycolysis, known as the Warburg effect, in hepatocellular carcinoma cells through interaction with pyruvate kinase M2 isoform (PKM2). This function underlines its involvement in cancer metabolism, as it attenuates colitis by regulating TGF-β signaling pathways. Furthermore, studies have linked TSP50 to cancer stem cell maintenance, driving stem-like properties in breast cancer cells, thereby facilitating tumor initiation and self-renewal capabilities. These characteristics highlight TSP50's potential as a target in therapeutic interventions for cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cancer/testis antigen 20; Probable threonine protease PRSS50; protease, serine, 50; Serine protease 50; testes specific protease 50; testes-specific protease 50; testicular tissue protein Li 210; Testis-specific protease-like protein 50
Gene Aliases: CT20; PRSS50; TSP50
UniProt ID: (Human) Q9UI38
Entrez Gene ID: (Human) 29122
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