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Gibco
Human WISP-1 Recombinant Protein, PeproTech®
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Product Details
120-18-20UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Mouse,
Rat
Expression System
E. coli
Amino acid sequence
TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN
Molecular weight
38.1 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 ug/ml.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
120-18-1MG will be provided as 2 x 500 µg (120-18-500UG).
Recombinant Human WISP-1 is a 38.1 kDa protein containing 345 amino acid residues. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCN family member 4; CTC-458A3.8; WISP-1; Wnt-1 inducible signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1 induced secreted protein 1; WNT1-inducible-signaling pathway protein 1
Gene Aliases: CCN4; WISP1; WISP1c; WISP1i; WISP1tc
UniProt ID: (Human) O95388
Entrez Gene ID: (Human) 8840
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