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Invitrogen
Human alpha-1 PDX Recombinant Protein
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Product Details
RP-070
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
E. coli
Molecular weight
46.7 kDa
Class
Recombinant
Type
Protein
Purity
>80%
Activity
For furin inhibition assays, cells should be treated with 8.0 - 20 µM human alpha-1 PDX recombinant protein for 1 hour. For in vitro assays, 0.1 - 2.0 µM human alpha-1 PDX recombinant protein should be used.
Conjugate
Form
Lyophilized
Concentration
2.5 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
Product Specific Information
RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 µM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 µM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays.
Molecular weight is approximately 46.7 kDa.
Target Information
Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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