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Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Mouse IP-10 should be dissolved in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Mouse IP-10 is also soluble in most buffered solutions. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Storage: Lyophilized mouse IP-10 should be stored at 2-8°C preferably desiccated. Following reconstitution, apportion into working aliquots and store at ≤-20⪚C. Avoid repeated freeze-thaw cycles.
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
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