Gibco

Mouse RELM beta Recombinant Protein, PeproTech®

Product Details

450-26B-25UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Immunostaining (IS)

View 1 publication 1 publication

Product Specifications

Species

Mouse

Published species

Mouse

Expression System

E. coli

Amino acid sequence

MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA

Molecular weight

18 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine RELMbeta is an 18.0 kDa protein, consisting of two identical 83 amino acid polypeptide chains linked by a single disulfide bond.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Adipose tissue is an energy reserve that is strictly regulated in animals. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic features of allergic asthma are enhanced in the absence of resistin-like molecule-beta.

Scientific reports

LeMessurier KS,Palipane M,Tiwary M,Gavin B,Samarasinghe AE

Fri May 04 00:00:00 EDT 2018

Opposing roles of nuclear receptor HNF4α isoforms in colitis and colitis-associated colon cancer.

eLife

Chellappa K,Deol P,Evans JR,Vuong LM,Chen G,Briançon N,Bolotin E,Lytle C,Nair MG,Sladek FM

450-26B was used in Enzyme-linked immunosorbent assay to conclude that HNF4α isoforms perform non-redundant functions in the colon under conditions of stress, underscoring the importance of tracking them both in colitis and colon cancer.

Wed May 11 00:00:00 EDT 2016

Murine guanylate cyclase C regulates colonic injury and inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Steinbrecher KA,Harmel-Laws E,Garin-Laflam MP,Mann EA,Bezerra LD,Hogan SP,Cohen MB

Wed Jun 15 00:00:00 EDT 2011

Murine guanylate cyclase C regulates colonic injury and inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Steinbrecher KA,Harmel-Laws E,Garin-Laflam MP,Mann EA,Bezerra LD,Hogan SP,Cohen MB

Wed Jun 15 00:00:00 EDT 2011

Murine guanylate cyclase C regulates colonic injury and inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Steinbrecher KA,Harmel-Laws E,Garin-Laflam MP,Mann EA,Bezerra LD,Hogan SP,Cohen MB

Wed Jun 15 00:00:00 EDT 2011

Murine guanylate cyclase C regulates colonic injury and inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Steinbrecher KA,Harmel-Laws E,Garin-Laflam MP,Mann EA,Bezerra LD,Hogan SP,Cohen MB

Wed Jun 15 00:00:00 EDT 2011

Intestinal epithelial cell secretion of RELM-beta protects against gastrointestinal worm infection.

The Journal of experimental medicine

Herbert DR,Yang JQ,Hogan SP,Groschwitz K,Khodoun M,Munitz A,Orekov T,Perkins C,Wang Q,Brombacher F,Urban JF,Rothenberg ME,Finkelman FD

Mon Dec 21 00:00:00 EST 2009

Alternatively activated macrophage-derived RELM-{alpha} is a negative regulator of type 2 inflammation in the lung.

The Journal of experimental medicine

Nair MG,Du Y,Perrigoue JG,Zaph C,Taylor JJ,Goldschmidt M,Swain GP,Yancopoulos GD,Valenzuela DM,Murphy A,Karow M,Stevens S,Pearce EJ,Artis D

Mon Apr 13 00:00:00 EDT 2009

Goblet cell-derived resistin-like molecule beta augments CD4+ T cell production of IFN-gamma and infection-induced intestinal inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Nair MG,Guild KJ,Du Y,Zaph C,Yancopoulos GD,Valenzuela DM,Murphy A,Stevens S,Karow M,Artis D

Wed Oct 01 00:00:00 EDT 2008

Resistin-like molecule-beta is an allergen-induced cytokine with inflammatory and remodeling activity in the murine lung.

American journal of physiology. Lung cellular and molecular physiology

Mishra A,Wang M,Schlotman J,Nikolaidis NM,DeBrosse CW,Karow ML,Rothenberg ME

Wed Aug 01 00:00:00 EDT 2007

Resistin-like molecule beta (RELMbeta/FIZZ2) is highly expressed in the ileum of SAMP1/YitFc mice and is associated with initiation of ileitis.

Journal of immunology (Baltimore, Md. : 1950)

Barnes SL,Vidrich A,Wang ML,Wu GD,Cominelli F,Rivera-Nieves J,Bamias G,Cohn SM

Thu Nov 15 00:00:00 EST 2007

Absence of bacterially induced RELMbeta reduces injury in the dextran sodium sulfate model of colitis.

The Journal of clinical investigation

McVay LD,Keilbaugh SA,Wong TM,Kierstein S,Shin ME,Lehrke M,Lefterova MI,Shifflett DE,Barnes SL,Cominelli F,Cohn SM,Hecht G,Lazar MA,Haczku A,Wu GD

Wed Nov 01 00:00:00 EST 2006

Absence of bacterially induced RELMbeta reduces injury in the dextran sodium sulfate model of colitis.

The Journal of clinical investigation

McVay LD,Keilbaugh SA,Wong TM,Kierstein S,Shin ME,Lehrke M,Lefterova MI,Shifflett DE,Barnes SL,Cominelli F,Cohn SM,Hecht G,Lazar MA,Haczku A,Wu GD

Wed Nov 01 00:00:00 EST 2006

Resistin concentrations in murine adipose tissue and serum measured by a new enzyme immunoassay.

Obesity (Silver Spring, Md.)

Fujinami A,Ohta K,Matsui H,Kitada N,Kitaura Y,Kawahara Y,Obayashi H,Kuno S,Nakamura N,Ohta M

Wed Feb 01 00:00:00 EST 2006

Regulation of RELM/FIZZ isoform expression by Cdx2 in response to innate and adaptive immune stimulation in the intestine.

American journal of physiology. Gastrointestinal and liver physiology

Wang ML,Shin ME,Knight PA,Artis D,Silberg DG,Suh E,Wu GD

Sun May 01 00:00:00 EDT 2005

Resistin-like molecule beta activates MAPKs, suppresses insulin signaling in hepatocytes, and induces diabetes, hyperlipidemia, and fatty liver in transgenic mice on a high fat diet.

The Journal of biological chemistry

Kushiyama A,Shojima N,Ogihara T,Inukai K,Sakoda H,Fujishiro M,Fukushima Y,Anai M,Ono H,Horike N,Viana AY,Uchijima Y,Nishiyama K,Shimosawa T,Fujita T,Katagiri H,Oka Y,Kurihara H,Asano T

Fri Dec 23 00:00:00 EST 2005

RELMbeta/FIZZ2 is a goblet cell-specific immune-effector molecule in the gastrointestinal tract.

Proceedings of the National Academy of Sciences of the United States of America

Artis D,Wang ML,Keilbaugh SA,He W,Brenes M,Swain GP,Knight PA,Donaldson DD,Lazar MA,Miller HR,Schad GA,Scott P,Wu GD

Tue Sep 14 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: Cysteine-rich secreted protein A12-beta; Cysteine-rich secreted protein FIZZ2; found in inflammatory zone 2; RELMbeta; Resistin-like beta; resistin-like molecule beta; RSNB

Mouse RELM beta Recombinant Protein, PeproTech®

Product Details

450-26B-25UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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