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Mouse Wnt-3a Recombinant Protein, PeproTech®
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Product Details
315-20-10UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Published species
Chicken,
Human,
Mouse,
Non-human primate,
Rat
Expression System
Mammalian cells
Amino acid sequence
SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK
Molecular weight
37.4 kDa
Class
Recombinant
Type
Protein
Purity
≥ 75% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<2 EU/µg
Activity
Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6 ng/ml.
Conjugate
Form
Lyophilized
Amount
10 µg
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Murine Wnt-3a is a monomeric glycoprotein containing 334 amino acid residues. Due to glycosylation, the Murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; vestigial tail; wingless-related MMTV integration site 3A; Wnt 3a; WNT3A protein
Gene Aliases: vt; Wnt-3a; Wnt3a
UniProt ID: (Mouse) P27467
Entrez Gene ID: (Mouse) 22416
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