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Invitrogen
Proteinase K Recombinant Protein
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Product Details
RP-87705
Product Specifications
Species
Fungi
Expression System
P. pastoris
Amino acid sequence
Full length Proteinase K protein
Class
Recombinant
Type
Protein
Purity
>95% by SDS-Page
Activity
Measured by its digestion activity. The activity is >30 U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Animal Origin Free
No DNase contamination detected by digestion of lambda DNA at 37°C for 6 hours. No RNases contamination detected by digestion of lambda DNA at 25°C for 16 hours. Host genomic DNA/RNA is no residual detected by PCR.
Storage: Store the lyophilized protein at 4°C for up to 1 year and less than -20°C for long term. Lyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. After reconstitution, the protein solution is stable at -20°C for 1 year, at 2-8°C for up to 6 months.
Target Information
Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 6°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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