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Invitrogen
Rat IL-17A ELISA Standard Recombinant Protein
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Product Details
39-8170-65
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Storage conditions
-80°C
Product Specific Information
Description: Interleukin-17A (IL-17A, also known as CTLA-8 and IL-17) is a member of the IL-17 family of proinflammatory cytokines. It is most similar to IL-17F, another member of the family with which it shares 50% sequence homology. Both cytokines exist as homodimers and individual subunits from each protein can also associate into a heterodimer known as IL-17AF.
Expression of IL-17A is most often associated with the Th17 subset of CD4^+ T cells, but has also been observed in CD8^+ T cells, gammadelta T cells, and neutrophils. IL-17A is important as a neutrophil activator, and it induces inflammatory cytokine production in epithelial cells, endothelial cells, and fibroblasts through NF-kappaB signaling. IL-17A and Th17 cells have been implicated in various autoimmune disorders.
Applications Reported: The intended use of the Recombinant Rat IL-17A Single-Use ELISA RSG Standard is as a standard for Rat IL-17A (homodimer) ELISA Ready-SET-Go!. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper dilution of the recombinant standard.
Applications Tested: This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the Rat IL-17A (homodimer) ELISA Ready-SET-Go!®.
Source: E. coli expressed amino acids Ala18-Ser150, accession number AAA37490.
Purity: >98% as determined by SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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