Shop All Expression mRNA and Pre-Made Viruses

LentiArray™ Human Apoptosis CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Apoptosis Library targets 904 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 3,616 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

Apoptosis is a tightly regulated process that is essential for maintaining homeostasis in multicellular organisms. Inhibition of apoptosis can result in the development of cancer, autoimmune, and inflammatory disease and is involved in viral infection. Conversely, over-activation of apoptosis can lead to atrophy, tissue damage, and neurodegenerative disease. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

GeneArt™ CRISPR Nuclease mRNA (Invitrogen™)

GeneArt™ CRISPR Nuclease mRNA is formulated using a new manufacturing process to achieve superior quality and improved product performance. This wild-type Cas9 mRNA has two nuclear localization signals (NLSs). Cas9 constructs with two NLSs have been shown to be more efficient at targeting the Cas9 protein to the nucleus (1). The mRNA format is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems. Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break. The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency. Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA. Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection.

How to obtain a gRNA sequence
Genome editing with CRISPR technology requires a noncoding guide RNA (gRNA) in order to cleave genomic DNA at a target sequence of interest. The gRNA has two molecular components: a target-specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) that have been combined into one transcript. The target sequence (20 bases) must be immediately upstream of a PAM motif (NGG) which allows the Cas9 to initiate binding. The PAM is only on the target DNA and not part of the target specific CRISPR sequence. The gRNA and the PAM motif guide the Cas9 nuclease to the target genomic sequence to form a complex and create a double-stranded blunt DNA break (DSB) three nucleotides upstream from the PAM site.

Use our GeneArt CRISPR Search and Design Tool to search our database of >600,000 gRNA sequences specific to every gene in the human and mouse genomes. GeneArt predesigned gRNAs are optimized for gene knockout and typically target the first three transcribed exons per gene. Search results include recommendations based on minimizing potential off-target effects, potential binding sites, and exon maps with gRNA locations. This tool can also be used to analyze any sequence of interest to design unique CRISPR sequences.

How to make gRNA
Once gRNA sequences have been selected, choose from three options for making gRNA:
1. The GeneArt Precision gRNA Synthesis Kit for transfection-ready gRNA in as little as four hours including template assembly.
2. GeneArt CRISPR Strings, a synthetic DNA template consisting of the gRNA with a T7 promoter for in vitro transcription or U6 promoter for ready-to-transfect format, which may be purchased via the GeneArt CRISPR Search & Design Tool or by completing the GeneArt CRISPR Strings DNA Order Form and submitting it to GeneArtSupport@lifetech.com.
3. Save time and effort and have our GeneArt custom services team design, synthesize, and purify in vitro transcribed (IVT) gRNA sequences for you. To obtain a services quotation, or to order, please contact our Custom Services department at 1-800-955-6288 x45682 or custom.services@lifetech.com.

Resources
Demonstrated protocol: Engineering stem cells with CRISPR-Cas9

References
1.Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339(6121):819-23.

LentiArray™ Human Cell Cycle CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Cell Cycle Library targets 1,444 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 5,776 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

Cell cycle regulators are important to normal development and also play a role in the development of cancer, cardiovascular, inflammatory, and neurodegenerative diseases. The gene targets within the LentiArray Human Cell Cycle Library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC), and include cyclin-dependent kinases, or CDKs, regulators of cell cycle progression such as CIP/KIP family proteins and members of the INK4 family of cell cycle inhibitors, proteins in the retinoblastoma family, and DNA replication factors such as the cell division cycle proteins (CDCs). The gRNA designs for each target were created using Thermo Fisher Scientific's proprietary gRNA design algorithm.

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

LentiArray™ CRISPR Negative Control Lentivirus, human, non-targeting, with GFP (Invitrogen™)

Negative control gRNAs are essential in screening applications for setting thresholds for hit determination. The Invitrogen™ LentiArray™ CRISPR Negative Control Lentivirus, Human Scrambled, contains a gRNA sequence with no sequence homology to any region of the human genome. This construct also expresses emGFP to provide a visual readout of successful transduction that can be used in determining Multiplicity of Infection (MOI).

BacMam LRRK2-GFP G2019S Reagent (Invitrogen™)

BacMam LRRK2-GFP G2019S Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length LRRK2-GFP G2019S in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2-GFP G2019S Reagent Lets You:

• Easily overexpress full-length LRRK2-GFP G2019S in most cellular backgrounds
• Visualize LRRK2 expression
• Conduct the LRRK2 cellular assays you need
• Understand the effects of therapeutically relevant LRRK2 mutations

Use BacMam Technology with GFP for Expression and Visualization of LRRK2
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2-GFP G2019S protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods, such as primary neurons and neuroblastoma cell lines.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2-GFP G2019S has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function) and identification of potential physiological substrates for LRRK2.

Examine LRRK2 Mutations with BacMam Reagents
Use therapeutic mutant LRRK2-GFP G2019S along with the wild-type LRRK2-GFP (Cat No A14170) reagent to enable your research into LRRK2 mutations, including exploration of differences in cellular response to stimulus among different mutations. Use LRRK2-GFP to confirm LRRK2-GFP protein expression and compare localization of wild type with pathological mutants in various cell backgrounds, especially primary neurons.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam LRRK2 Reagent (Invitrogen™)

BacMam LRRK2 Wild Type Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length wild-type LRRK2 in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 Wild Type Reagent Lets You:

• Easily overexpress full-length LRRK2 in most cellular backgrounds
• Conduct the LRRK2 cellular assays you need
• Understand the effects of therapeutically relevant LRRK2 mutations

Examine LRRK2 Mutations with BacMam Reagents
Use therapeutic mutant reagents G2019S (Cat. no. A13389) and D1994A (Cat. no. A13390) along with the wild-type LRRK2 reagent to enable your research into LRRK2 mutations, including exploration of differences in cellular response to stimulus among different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function), and identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods, such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

BacMam Histone H3 Reagent (Invitrogen™)

The BacMam Histone H3 Reagent provides a convenient genetic delivery tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. The GFP fusion enables sensitive detection of post-translational modifications using LanthaScreen® TR-FRET Cellular Assay technology.

The BacMam Histone H3 Reagent Lets You:

• Use your cell background of choice with the portability of BacMam
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The BacMam Histone H3 allows the investigation of post-translational modifications of histone H3 in your choice of cellular backgrounds, including primary cells. This enables screening for potential inhibitors of histone-modifying enzymes such as kinases, acetylases, deacetylases, methyl transferases, and demethylases in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen® Convenience Saves Sample and Time
When used with a LanthaScreen® Tb-anti-Histone H3 antibody against a specific modification, assays can be run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA. By using the same BacMam reagent for GFP-Histone H3, a variety of histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen® technology includes all of the advantages of TR-FRET detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells.

For research use only. Not for human or animal therapeutic or diagnostic use.

LentiArray™ Human Drug Transporter CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Drug Transporter Library targets 98 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 392 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

Transporter proteins regulate the movement of molecules across cellular membranes and play a key role in pharmacology. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

LentiArray™ Human Cell Surface CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Cell Surface Protein Library targets 778 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 3,112 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

The broad array of cell surface proteins allows the cell to receive information from and react to its environment. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

LentiArray™ Human Ubiquitin CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Ubiquitin Library targets 943 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 3,722 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

The ubiquitin system is integral to maintaining cellular homeostasis by regulating protein turnover. Dysregulation of the ubiquitin system has been linked to multiple diseases, including cancer, neurodegenerative, musculoskeletal, cardiovascular, and metabolic diseases, as well as being linked to viral infection. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

LentiArray™ CRISPR Positive Control Lentivirus, human HPRT (Invitrogen™)

Optimizing delivery conditions is a critical step in any experiment utilizing the CRISPR/Cas9 system. The Invitrogen™ LentiArray™ CRISPR Positive Control Lentivirus gRNA against HPRT is a highly efficient gRNA validated in multiple human cell lines that has achieved editing efficiencies of greater than 80%. It can be used to rapidly determine ideal delivery conditions in order to achieve maximum editing efficiency in your cell model of choice.

LentiArray™ Cas9 Lentivirus (Invitrogen™)

LentiArray Cas9 Lentivirus provides an efficient method to drive high level Cas9 nuclease expression in a wide variety of cell types. The LentiArray Cas9 Lentivirus construct includes a human codon-optimized version of Cas9 with two nuclear localization signals to facilitate efficient delivery into the nucleus. The 100 µL size is sufficient for creating stable Cas9 expressing pools of cells or for isolating stable Cas9 expressing clones. LentiArray Cas9 Lentivirus is provided as ready to use lentivirus at a titer of 1 x 107 TU/mL.

LentiArray™ Human Epigenetics CRISPR Library (Invitrogen™)

The award-winning Invitrogen™ LentiArray™ Human Epigenetics Library targets 396 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 1,548 gRNAs. Libraries are delivered as 200 μL of ready to use lentiviral particles per gene target with an average titer of 1x108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks.

Epigenetic regulation of gene expression plays a central role in normal development and is being recognized as a contributing factor to the development of many diseases. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC).

Built to bring you success
LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm. This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs. The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types.

Design and execute your experiments without limitations
LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells.

The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter. You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line. With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals.

Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers. This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments. For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes.

Learn more about LentiArray CRISPR libraries >

Optimize your assay design and have full confidence in your results with LentiArray controls
High quality controls are the cornerstone of a successful screen. The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.

Learn more about LentiArray controls >

CytoTune™ EmGFP Sendai Fluorescence Reporter (Invitrogen™)

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction.

The CytoTune™ EmGFP Sendai Fluorescence Reporter enables a better understanding of how both the original CytoTune™-iPS Sendai Reprogramming kits and the newer CytoTune™-iPS 2.0 Sendai Reprogramming Kit transduces various cell types. The Sendai particle enters a cell via its HN envelope protein that recognizes sialic acid and is widely expressed in mammalian cells, allowing Sendai virus to target a wide range of cell types. The Sendai virus vectors do not enter the nucleus, thus avoiding any DNA phase, as the viral genome remains as RNA in the cytoplasm.

BacMam LRRK2 G2019S Reagent (Invitrogen™)

BacMam LRRK2 G2019S Reagent is a modified insect cell virus (baculovirus) that enables easy, efficient, and titratable overexpression of full-length G2019S LRRK2 upregulating mutation in your choice of cellular background, including primary neurons and neuroblastoma cell lines.

BacMam LRRK2 G2019S Reagent Lets You:

• Easily overexpress full-length LRRK2 G2019S in most cellular backgrounds
• Understand the effects of therapeutically relevant LRRK2 mutations
• Conduct the LRRK2 cellular assays you need

Examine LRRK2 Mutations with BacMam Reagents
Using the BacMam LRRK2 therapeutic mutant reagents G2019S and D1994A (Cat. no. A13390) along with the wild-type LRRK2 reagent (Cat. no. A13388) enables you to research and determine LRRK2 mutant mechanisms. You can also evaluate the difference in the cellular response to a stimulus among the different mutations.

Expand Your Battery of Cellular Assays for LRRK2
Once full length LRRK2 G2019S has been introduced into your cellular background, the transduced cells allow you the flexibility to conduct many types of LRRK2 experiments, including studies of regulation of physiologically functional pathways (including signaling pathways leading to function), and identification of potential physiological substrates for LRRK2.

Overexpress Full-Length LRRK2 in Most Mammalian Cell Backgrounds
BacMam technology utilizes a modified baculovirus to deliver and express genes in mammalian cells with minimal effort and toxicity. It enables efficient, titratable LRRK2 G2019S protein expression in various mammalian cellular backgrounds, including cell lines that may not be compatible with traditional transfection methods such as primary neurons and neuroblastoma cell lines.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology