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View additional product information for Pierce™ Bradford Plus Protein Assay Reagent - FAQs (23238)
4 product FAQs found
Pierce Bradford Plus Protein Assay Reagent is a quick and ready-to-use modification of the well-known Bradford, Coomassie dye-binding, colorimetric method for total protein quantitation. This Bradford reagent modification greatly reduces the tendency of Coomassie dye-containing reagents to give a characteristically nonlinear response curve. Pierce Bradford Plus Protein Assay Reagent results in a substantially more linear response curve in a defined range of protein concentration. Thermo Scientific's special formulation results in significantly less protein-to-protein variation than that which is observed with other Bradford-type Coomassie dye-based formulations. When Coomassie dye binds protein in an acidic medium, an immediate absorbance shift occurs from 465 nm to 595 nm, with a simultaneous color change of the reagent from green/brown (or red/brown) to blue.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
You can remove interfering substances easily with the use of one of several Thermo Scientific products designed to purify your sample. These products include Slide-A-Lyzer Dialysis Cassettes, D-Salt Desalting Columns and Extracti-Gel D Detergent Removing Gel. Furthermore, TCA and cold acetone can be utilized to remove other interfering substances. (See the TechTips section of this website for more information.)
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Care must be exercised when cleaning glassware that will be used again for protein assays. Thorough cleaning often requires the use of a detergent but it must be completely removed in the final rinse. Also, the Coomassie dye will stain glass or quartz cuvettes. The cuvettes can be cleaned easily with our detergents followed by a thorough final rinsing with deionized water. If you prefer, disposable polystyrene cuvettes may be used to eliminate the cuvette-cleaning chore.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
If a photometer or plate reader is not available with a 595 nm filter, the blue color may be measured at any wavelength between 570 nm and 610 nm. The maximum sensitivity of the assay occurs when the absorbance of the Coomassie dye protein complex is measured at 595 nm. Taking the absorbance measurements at any wavelength other than 595 nm will result in a lower slope for the standard curve and may increase the minimum detection level for the protocol.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.