Next-Generation Sequencing for Lymphoid Genomic Profiling

The need for more comprehensive lymphoid testing is growing

As the list of known mutations and relevant genes for lymphoid disorders increases, characterizing these samples becomes more complicated and time-consuming. To get meaningful genetic insights, you need a rapid and efficient approach for evaluating all key mutations upfront. Next-generation sequencing (NGS) can streamline the path from sample to result by providing a comprehensive molecular profile with speed and simplicity.

On Demand Webinar: Utility of NGS in lymphoid malignancies and Ion AmpliSeq Liverpool Lymphoid Network Panel experience

NGS has become a key component of molecular testing in both myeloid and lymphoproliferative neoplasms. It can facilitate improvements in classification, prognostication and identification of therapeutic targets. In this presentation, hear our expert share the utility of faster molecular results in lymphoid genomic profiling and their experience with the Genexus System and the Ion AmpliSeq Liverpool Lymphoid Network Panel.

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Dr. Michael Krigstein

Consultant Haematologist MBBS(Hons) FRACP FRCPA MCncrSc

SydPath, St Vincent’s Hospital

Sydney, Australia

On Demand Webinar: Implementing NGS for the study of lymphoid malignancies

In this on-demand webinar, Dr. Gerard Frigola discusses his experience with the new Ion AmpliSeq Liverpool Lymphoid Network Panel and shares compelling analytical data from his lab to demonstrate the impact of NGS for lymphoid
malignancy research.

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Gerard Frigola, MD

Hematopathologist

Hospital Clinic, IDIBAPS

University of Barcelona

Ion AmpliSeq Liverpool Lymphoid Network Panel benefits

The Ion AmpliSeq Liverpool Lymphoid Network Panel is a community panel designed by leading clinical researchers for more comprehensive lymphoid genomic profiling.

Rapid	Automated	Comprehensive	Verified performance	Community network
Go from specimen to result in as little as one day	Process samples with minimal hands-on time, available on both GeneStudio & Genexus	Simultaneously profile 60 key DNA mutations relevant for a broad range of lymphoid disorders	Reliably detect variants using a panel designed and tested by community users with real-world clinical research samples	Access a community network of labs and contribute to future iterations of the panel design

Profile 60 key DNA mutations with one test

Panel content has been curated to cover relevant targets for T-cell and B-cell lymphomas, leukemias and other lymphoproliferative disorders. It is compatible with all common sample types, including blood, bone marrow and formalin-fixed paraffin embedded (FFPE) tissue.

Ion AmpliSeq panel design includes:

Essential exons and full coding sequence coverage of key genes
3’ UTR coverage of NOTCH1
Non-coding exon 1 of BCL6 and partial intron 1

Essential exons (36)		Full coding sequence (24)
BCL6	IRF4	ARID1A	PAX5
BIRC3	KRAS	ATM	PIM1
BRAF	MAP2K1	B2M	PRDM1
BTK	MYD88	BCL2	PTEN
CARD11	NOTCH1	CDKN2A	SAMHD1
CCND1	NOTCH2	CREBBP	SGK1
CCND3	NRAS	DIS3	SOCS1
CD79B	PLCG2	GNA13	TENT5C
CXCR4	POT1	ID3	TET2
DNMT3A	RHOA	KMT2D	TNFAIP3
EP300	RPS15	MEF2B	TP53
ETV6	SF3B1	MYC
EZH2	SMARCA4	NFKBIE
FAS	STAT3
FBXW7	STAT5B
FOXO1	STAT6
HRAS	TNFRSF14
IDH2	XPO1

Oncomine Lymphoma Panel

The Oncomine Lymphoma Panel contains 25 key genes associated with all major B-cell lymphomas, including DLBCL, follicular lymphoma, mantle cell lymphoma, and marginal zone lymphomas. This panel provides robust performance with as little as 10 ng of input and comes with an end-to-end, clinical research-grade workflow including straightforward, customizable report that summarizes the findings. It is available for the Ion Chef and Ion GeneStudio S5 systems. Customize the assay to suit your needs by selecting from a library of primer designs to add additional gene targets.

Lymphoma
ARID1A	BRAF	CDKN2A	HIST1H1E	MYD88	TNFAIP3
ATM	BTK	CREBBP	KMT2D	PIM1	TNFRSF14
B2M	CARD11	EZH2	MTOR	SOCS1	TP53
BCL2	CD79B	GNA13	MYC	SF3B1	XPO1
BCL6

NGS systems

The Oncomine Comprehensive Assay v3 is available on the Genexus System and additionally available for use on the Ion GeneStudio S5 System with manual or automated library preparations.