System overview



Visibly simple food pathogen testing—simple workflows designed to get results.

Rapidly and accurately detect foodborne pathogens, and other important food safety and quality targets with the Thermo Scientific SureTect Food Safety PCR System.

Empower your operations with simple workflows and rapid reporting to enable faster product release and interventions. Our food safety PCR assays offer detection of the major pathogens and other important targets in food, animal feed, primary production samples, and samples from environmental manufacturing.

Whether your laboratory is looking to introduce PCR technology for the first time, or you want to expand the PCR testing services you offer, we can help you find the right solution.

Streamlined workflow


Streamlined workflow graphic

SureTect PCR Assays


Validated for a broad range of targets and matrices according to ISO16140-2:2016 and AOAC validation and certification requirements, the SureTect PCR Assays provide the ultimate solution for all your foodborne pathogen detection requirements.

  • Pre-dispensed lysis and PCR reagents, reducing handling steps and risk of operational error
  • NEW color-coded frames for lysis and PCR stripe well plates for easy assay target identification
  • NEW clearly oriented lysis and assay plates reducing human error
  • Optimized liquid lysis step with pre-dispensed reagents
  • NEW color indicator in Proteinase K Reagent enabling tracking of pipetted wells
  • Thin-walled polypropylene wells ensure superior thermal cycling performance for consistent results run after run
  • Internal amplification control (IAC) in every well providing confidence in every result
  • NEW pierceable seals to reduce handling steps
Suretect PCR assays kit
Color-coded Armadillo strip-well plates

 Color-coded Assay Strip Plates

Easy, safe separation into 8-well strips

Easy, safe separation of Lysis and Assay plates into 8-well strips

Cut strips to required number of tubes

Cut strips to required number of tubes

Color-indicator in Proteinase K

Color-indicator in Proteinase K Reagent

Efficient plate sealing

Efficient plate sealing

Pierceable lysis tube seals

Pierceable lysis tube seals

Instruments & software


Applied BioSystems™ QuantStudio™ 5 Food Safety Real-Time PCR System

The SureTect PCR Assays are validated to run on the QuantStudio 5 Food Safety PCR system using Thermo Scientific RapidFinder Analysis Software for easy test set-up, results interpretation and data analysis.

The QuantStudio 5 Food Safety PCR System is a high-performance benchtop platform, combining ease-of-use and system connectivity with powerful options that enable maximum data utility and control. Benefit from the flexibility of a 6-channel, 96-well, cloud-enabled open platform suitable for running our full range of PCR solutions for food safety, authenticity, and quality testing.

Intuitive and easy-to-use for all levels of experience with touchscreen usability, allowing you to stay connected to your data easily.

  • Multiplex with ease—up to six excitation and six emission filters offer 21 different color combinations, allowing a broad range of detection chemistries and maximum multiplexing
  • Get up and running quickly—factory-calibrated for accuracy, quick installation, and immediate use; pre-optimized protocol templates help minimize training for new users
  • Maximize benchtop space—compact instrument can be configured as a stand-alone or with a computer
  • Open instrument—suitable for all our food safety, authenticity, and quality PCR tests
  • Intuitive, user-friendly software, avoiding subjective interpretation
  • Cloud-enabled, offering the potential for data sharing, analysis, and trending
  • Capacity for processing several hundred samples a day

Order the QuantStudio 5 Food Safety PCR System

Thermo Scientific RapidFinder Analysis Software v2.0

An integral part of the SureTect workflow, the RapidFinder Analysis Software 2.0 is a graphical, easy-to-use tool for food and environmental testing labs to optimize their pathogen detection workflow.

Simple set-up

  • Intuitive sample set up
  • Flexible plate layout including automatic or customisable options
  • Pre-set, optimized thermal cycling conditions per target

Automatic data interpretation

  • Fully automated amplification, detection, data collection, and data analysis
  • Quick interpretation of data with flags, notifications, and prompts
  • Detailed report generation for printing or export
  • Interoperability with LIMS or similar data management tool for importing plate set-up and exporting assay results
  • Automatically generates results (presence or absence of the target)
  • Offers interrogation of results for the whole plate, grouped by sample, or for individual PCR tubes

Order the RapidFinder Analysis Software v2.0

Handling tools


Easy-to-use tools to simplify your workflow: designed specifically to enhance each step of the SureTect PCR Assay workflow, the new Thermo Scientific™ SureTect Tools enable ease of handling, reducing hands-on time and human error, providing a superior user handling experience.

 ProductDescriptionCat. No.

PCR Strip Cutting Tool

Cut Lysis and PCR Assay Strips when fewer than 8 tubes are needed.


PCR Lysis Tube Uncapping Tool

For removal of Domed Strip Caps from Lysis Strips.


PCR Capping Tool

For capping Lysis and PCR Assay Plate strips.


96-well PCR Base

96-well base for Lysis and PCR Assay Strip Plates. Use in Lysis and PCR steps.


MicroAmp adhesive film applicator

Pack of 5; for ease of applying the Pierceable Film to Lysis Plate strips


PCR Strip Cutting Tool

Capping Tool with Domed Lysis Tube Caps

Uncapping Tool with Domed Lysis Tube Caps

SureTect PCR Workflow video

SureTect Trays


ProductDescriptionCat. No.

SimpliAmp Strip-Plate Tray

For compatibility and ease of loading SureTect Lysis Tubes into the SimpliAmp Thermal Cycler


QuantStudio 5 Strip-Plate Tray

For compatibility and ease of loading SureTect PCR Assay Tubes into the QuantStudio 5 PCR instrument


ABI 7500 Fast Precision Plate Holder

For compatibility and ease of loading SureTect PCR Assay Tubes into the 7500 FAST PCR instrument


SimpliAmp Strip Plate Tray Cover

Pack of 10 For covering and securing the Lysis Tubes before pipetting




Each SureTect PCR Assay comes with all the reagents and PCR accessories needed to set up your SureTect PCR Assay tests to run on the QuantStudio 5 Food Safety PCR System. Additional SureTect Accessories are available to order separately, including Armadillo low-profile PCR strip plates for balancing when using the Applied Biosystems 7500 Fast Food Safety PCR System and domed lysis tube caps for use if preferred in place of the pierceable lysis seals.

ProductDescriptionCat. No.

Pierceable lysis seal

Pack of 100 seals

  • 2 included with each kit
  • Alternative option to lysis caps
  • Printings around each well for positioning and orientation
  • Strip perforations

Lysis tube caps, domed, pack of 120 strips

Pack of 120 strips: Optional domed caps that can be used in place of pierceable seals for the Lysis Tubes


Armadillo Low-Profile PCR Strip Plate, 96 well, clear

For balancing of 7500FAST plates


Ultra clear qPCR caps

Additional PCR caps, if required


SureTect workflow improvement starter pack


Get up and running quickly and easily with the new, improved SureTect PCR Assay workflow with the convenient SureTect Worfklow Starter Pack containing all of the tools and accessories you need.

ProductDescriptionCat. No.

SureTect Workflow Starter Pack

Contains: 1x PCR Strip Cutting Tool, 1x PCR Strip Capping Tool, 1x PCR Strip Uncapping Tool, 2x 96-well PCR Base, 1x SimpliAmp Strip Plate Tray, 1x QuantStudio 5 Strip Plate Tray, 1x ABI 7500 Fast Precision Plate Holder, 1x SimpliAmp strip plate tray cover, 1x MicroAmp Adhesive Film Applicator and 120x Lysis tube caps–domed


Validation & certification


SureTect PCR Assay validated workflows

SureTect PCR Assay workflows are validated for a broad range of matrices according to ISO 16140-2:2016 (AFNOR VALIDATION) and AOAC and fully supported by our dedicated and experienced technical teams.

View certifications



SureTect system

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